A mouse somatostatin (SS) receptor cDNA was cloned from neuroblastoma x glioma (NGI08-15) cells. The sequence is almost identical to that of the mouse SgTR2 r~eeptor [(1992) Proc, Nail, Acad, Sci, USA 89, 251)l bat lacks about 300 nu01eotides between transmembran¢ domain VII and the C-terminus. This spliced variant of SSTR2 (designated SSTR2B) encodes a protein which is 23 residues shorter than that predicted from the SSTR2 sequence, and differs in 15 amino acids at the C-terminus. mRNA corresponding to SSTR2B occurs in mouse tissues in higher abundance than that of SSTR2, SSTR2B binds SS peptides with high aflinit), when expressed in mammalian cells,

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A novel G-protein-coupled receptor was isolated from mouse and rat neuronal and lymphatic tissues. The amino acid sequence of the rat receptor (rNLR) shows an overall homology of 80% to a recently cloned receptor from Burkitt's lymphoma cells (BLRl) which is exclusively expressed in lymphatic tissues [(1992) Eur. J. Immuno1. 22, 27951. Much less homology between rNLR and BLRl was observed at the N-terminus (about 40%), whereas rNLR and the mouse homologue mNLR show 92% amino acid identity. Northern blot analysis of NLR revealed a predominant 5.5 kb mRNA species in various brain regions and neuronal cell lines, whereas in the spleen a 3 kb transcript is predominant. This distribution suggests a role of NLR in the nervous and immune systems.

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