Introduction: Thyroid hormones are required for normal development as well as regulating metabolism in the adult. A decreased thyroid hormone adversely affects erythropoietic system causes anemia. Our objective was to assess the relationship between serum vitamin B12 levels and thyroid function in anemic pregnant women. Aim: The Aim and objective of the study was to compare the vitamin B12 with thyroid hormones in anemic pregnant women. Materials & Methods: Study consisted of 50 subjects from OPD/IPD Index Medical College & Research Center, INDORE, MADHYA PRADESH, India. The study groups were divided into Group I-25 anemic pregnant women and Group II-25 Non – anemic pregnant women. The serum samples were collected from each subject and levels of different biochemical parameters were estimated. Result:  were noted. On comparing values in vitamin B 12 and thyroid hormones , among two groups, a significant difference (p<0.005) was found between few of them. Conclusion:  Screening of vitamin B12 levels in first trimester itself will be beneficial to prevent the complications of pregnancy. Further, hypothyroidism also present. So, early diagnosis of these deficiencies will be useful to start giving supplements to avoid unwanted effects in pregnancy.