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This study evaluated the protective effect of TiF4 and chitosan toothpaste on erosive tooth wear (ETW) in vitro. Enamel and dentin samples were randomly assigned to toothpastes (n = 12): (G1) TiF4 (1400 ppm F−), (G2) 0.5% chitosan (75% deacetylation, 500 mPas), (G3) TiF4 (1400 ppm F−) plus 0.5% chitosan (75% deacetylation, 500 mPas), (G4) Placebo, (G5) Erosion Protection (Elmex-GABA, 1400 ppm F−). Twelve samples were only eroded. All samples were submitted to erosive pH cycles and G1 to G5 to abrasive challenges using toothpastes’ slurries plus 45 s of treatment, for 7 days. The final profile was overlaid to the baseline one for the ETW calculation (µm). The data were subjected to Kruskal–Wallis/Dunn tests. TiF4 toothpastes, regardless of the presence of chitosan, were able to significantly reduce ETW compared to placebo, while chitosan alone was similar to placebo for both tissues. The toothpastes containing TiF4 were even superior to the commercial Elmex toothpaste on enamel, while they were similar on dentin; both were also significantly different from placebo for both tissues. TiF4 and Elmex toothpastes minimized the impact of brushing on eroded surface. In conclusion, TiF4 toothpastes, regardless the presence of chitosan, showed to be effective in minimizing ETW in vitro.

The effect of solutions containing a statherin-derived peptide (Stn15pSpS) on the protection against enamel erosion in vitro was evaluated. Bovine enamel specimens were divided into 4 groups (n = 15/group): 1) Deionized water (negative control), 2) Elmex Erosion Protection™ (positive control), 3) 1.88 × 10-5 M Stn15pSpS and 4) 3.76 × 10-5 M Stn15pSpS. The solutions were applied on the specimens for 1 min. Stimulated saliva was collected from 3 donors and used to form a 2-h acquired pellicle on the specimens. Then, the specimens were submitted to an erosive pH-cycling protocol 4 times/day, for 7 days (0.01 M HCl pH 2.0/45 s, artificial saliva/2 h, and artificial saliva overnight). The solutions were applied again during pH cycling, 2 times/day for 1 min after the first and last erosive challenges. Enamel loss (µm) was assessed by contact profilometry. Data were analyzed by Kruskal-Wallis and Dunn's test (p < 0.05). The best protection against erosion was conferred by Elmex Erosion Protection that significantly differed from all the other treatments, followed by the solutions containing Stn15pSpS, regardless of the concentration. However, 3.76 × 10-5 M Stn15pSpS did not differ from the negative control. The solution containing the lower concentration of Stn15pSpS protected against erosion in vitro, which should be confirmed using protocols that more closely resemble the clinical condition.

Objetivo: Avaliar a interferência do tratamento com extrato de Casearia Sylvestris Sw. sobre a angiogênese induzida por células tumorais de Erhlich. Métodos: Utilizou-se 28 ovos embrionados aleatoriamente distribuídos em quatro grupos de ovos (n=7/grupo), sendo eles: Grupo 1 - Soro fisiológico; Grupo 2 - Tumor de Ehrlich (TE); Grupo 3 - Casearia Sylvestris Sw. - Grupo 4: Casearia Sylvestris Sw + Tumor de Ehrlich. Os ovos foram submetidos à incubação durante 48 horas; sendo sujeitados ao tratamento decorridas 24 horas iniciais de incubação. Resultados: O tipo de tratamento aplicado em cada grupo interferiu individualmente nos resultados, onde relatou-se: Grupo 1- Padrão normal de crescimento (grupo controle); Grupo 2 - Aumento na proliferação de vasos sanguíneos; Grupo 3 - Baixa interferência no efeito do tratamento; Grupo 4 - Ramificações de vasos sanguíneos menos evidentes. Conclusão: Considera-se através dessa técnica a possibilidade da utilização do extrato de Casearia Sylvestris Sw. para o tratamento de células tumorais de Ehrlich, pela eficácia da planta contra proliferação de células malignas, entretanto devem ser realizados testes confirmatórios para confirmar quais propriedades da mesma são responsáveis por tal efeito.

The protective effect of toothpastes containing TiF4 and chitosan on erosive tooth wear in vitroThis study evaluated the protective effect of the experimental TiF4 and chitosan toothpaste on erosive tooth wear (ETW) in vitro. Seventy-two bovine enamel and 72 root dentin samples were embedded, polished and subjected to the baseline profile analysis. The samples were protected in 2/3 of the surface and randomly assigned to 5 toothpastes (n=12): 1) Experimental containing TiF4 (1400 ppm F -, pH 3.08), 2) 0.5%.