Miniaturized, high throughput detection technologies including microfluidics systems represent powerful tools for biomarker discovery and analysis. Optimiser™ microplate technology combines microfluidics technology with standard SBS-configured 96-well microplate architecture and allows for the improvement of ELISA workflows. In this review, we present the "standard" and "high sensitivity" capabilities of the Optimiser™ ELISA in detecting human cytokine biomarker; IL-4, resulting in improving sensitivity by 1000-fold higher than the typical conventional ELISA. The Optimiser™ ELISA microplate employs standard ELISA equipment and protocols, selectively reduces sample volume 10-20 fold (Static Mode) and has the capability to amplify assay sensitivity by 1,000 fold (Repetitive Loading Flow-Through Mode) relative to conventional high sensitivity ELISA approaches. Optimiser™ allows sensitive and quantitative detection of biomarkers with demonstrated reproducibility, speed and linearity. In this study we demonstrate the utilization of Optmiser TM in the amplification of low-concentration markers. Optimiser™ microfluidicbased technology represents a revolutionary advancement in ELISA technology, and holds great promise for accurate, sensitive detection of novel biomarkers and its potential applications in clinical diagnosis.