Objectives:The aim of the present study was to evaluate the use of platelet rich fibrin and Nano-hydroxyapatite as a regenerative treatment in furcation class II defect. Material and methods: Eight young adult dogs was selected for the study; in each dog, class II furcation defects were surgically created in 4 premolars and were treated as follow: First was treated with Nano hydroxyapatite alone ; Second with PRF alone ; Third with Nano hydroxyapatite and PRF ; Fourth did not receive any additional treatment than debridement (controls). Results: histomorphometric analysis revealed that, the greatest amount of newly formed bone was observed in the (PRF+ NHA bone) group, followed by the PRF group, then the NHA group, with the least recorded in the control group. The difference was not statistically significant except in comparing (PRF+ NHA group) with control group (p=0.0035). Conclusion:The combined use of both PRF and Nano hydroxyapatite in treatment of furcation class II defects could accelerate healing processes, getting use of the synergic effect of the powerful inherent regenerative potential of PRF and Nano hydroxyapatite.