Reconstituted myoglobins (Mb) with iron and cobalt complexes of chlorins were prepared for the first time. The Fe(II)–Mb’s form oxy-adducts, but the reversibility of oxygen binding is rather poor. The Co(II)–Mb’s prepared under anaerobic conditions bind O2 reversibly. Reconstitution under aerobic conditions leads to Co(III)–myoglobins; Co(II) proteins derived therefrom undergo instantaneous oxidation back to the Co(III) derivatives.
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