Introduction:Early blight is a common disease of tomato, which is caused by Alternaria solani. Objectives:This work was accompanied to find an alternative to chemical fungicides and to screen tomato varieties against Alternaria solani. Methods:The infected leaves were collected from five tomato fields of Shere-e-Bangla Agricultural University, Dhaka and were cultured for the identification of the infectious fungus and The phytobiocidal role of six plants against Alternaria solani was evaluated in vitro model. Results & Discussion:Alternaria solani was identified as the infectious fungus. The growth of the test fungi Trichoderma spp. viz., Trichoderma viride, T. harzianum collected form NAMDEC and Trichoderma sp collected from field of BCSIR was monitored as optimum P H . All the selected Trichoderma spp. were antagonistic to A. solani. Antagonistic capacity of the Trichoderma spp. was tested by dual culture, volatile as well as non-volatile method. It was observed, T. viride was most effective in the reduction process of A. solani and T. harzianum. T. viride also showed highest inhibition in volatile and non-volatile trials. Six plant extracts viz., Adhatoda vasica (Nees), Azadirachta indica (A Juss). Ocimujm sanctum (L), Allium sativum (L), Datura metal (Linn) and Zingiber officinale (Rose) were selected to evaluate their in vitro efficacy of 5%, 10% and 20% concentration against the A. solani. Allium sativum was the most effective one against A. solani, followed by Azadirachta indica. The efficacy of five fungicides viz., Bavistin 50WP, Mancozeb 80WP, Indofil M-45, Sulcox 50WP and Tall 25EC were evaluated for their fungitoxicity against the A. solani at 100, 200,100, 600 and 800 ppm. Tall 25EC was the most effective fungicide against Alternaria solani followed by Mancozeb 80WP. After screening the five tomato varieties against A. solani, it was revealed that BARI Tomato-9 had the highest Percentage of Disease Index (PDI) and the leaf of BARI Tomato-7 had the lowest Percentage of Disease Index (PDI). Conclusion:The extract of Allium sativum was effective to control Alternaria solani at prescribed concentration. The highest PDI was found in BARI tomato-9 against Alternaria solani.

Introduction: The aim of this study was to assess the induction of solvents on the total phenol and flavonoid content and also the antioxidant activity of Ganoderma lucidum extracts. Materials & Methods: In this study, two concentrations (100% and 75%) of diethyl ether, ethanol, butanol, chloroform, and acetone were used as extractants of Ganoderma lucidum. Total phenol and flavonoid contents were measured by spectrophotometric methods and 1, 1-diphenyl-2-picrylhydrazyl (DPPH). Free radical scavenging assay was used for the investigation of antioxidant activity. Results & Discussion: Extractants significantly affected the % yield of extract, the quantity of phenol and flavonoids and antioxidant activity of Ganoderma lucidum mushroom. The highest extraction yield, around 38%, was achieved by 75% acetone, followed by 100% acetone (about 36%) and 75% chloroform (approximate 21%). Hydro-acetone extract exhibited the most significant antioxidative properties (EC50 value; 645.55 µg/mL) comprised of a higher total of phenol content. In conclusion, the total phenol content encouraged the antioxidative potential of Ganoderma lucidum mushroom. Conclusion: These findings indicate that the selective extraction of Ganoderma lucidum shows significant biological activities.

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Objective: The current study is designed to evaluate the cyto-depressive effects of Ganoderma lucidum (GL) and Pleurotus ostreatus (PO) and their binary amalgams (GL+PO) on meristematic cells of Allium cepa L. Methods: Experiments were assessed by utilizing A. cepa bioassay. Results: Onion knobs were exposed to 500 μg/mL, 1000 μg/mL, and 2000 μg/mL groupings of the concentrates for naturally visible and minute examination. The concentrates essentially repressed the root development of A. cepa L. contrasted with the control in a period of treatment and concentration-dependent manner (p<0.05). In addition, photomicrographs revealed chromosomal aberrations extending from c-mitosis, stickiness, and bridges in the root tip meristematic cells of A. cepa. All mushroom extracts demonstrated remarkable cyto-depressive impacts on meristematic cells of A. cepa L. with some changeability. Conclusion: In view of the results come out, GL and PO could be considered as potential sources of anticancer mixes. Notwithstanding, additionally thinks about are essential for disengagement and portrayal of the bioactive mixes and more examination on their pharmacological properties is required. It is first report in Bangladesh where we studied cyto-depressive role of edible mushrooms, Ganoderma lucidum (GL) and Pleurotus ostreatus (PO).