The objective of this research is to prepare high surface area activated carbon (AC) from agricultural biomass materials–pine nut shells–by the method of carbonization and alkaline activation. Adsorption isotherms of creatinine (CR) by pine nut shell-based high surface area activated carbon (AC) from aqueous medium have been studied. The results have been found that samples have larger capacity for removing CR from solution. The adsorption capacity of CR as intensity at first 50 min. After 50 min, it’s smooth and steady. The carbons prepared with KOH could be effectively used for the removal of CR.