Background: Empirically, breadfruit (Artocarpusaltilis) leaves have been used as traditional medicine for several diseases, such as acute renal failure. This study aimed to find the effects of breadfruit leaves infusion on blood creatinine levels in rats to model acute renal failure. Methods: This laboratory experimental study was conducted by using 30 male wistar rats, weighing150-250 g. The rats were divided into 5 groups. The first group is a negative control and the second group is a positive control. Groups 2-5 were inducedby Gentamicine and peroxicam for 7 days. Groups 3-5 were given infusion of breadfruit leaves in different dosage for 7 days then the blood creatinine levels were tested at the 15th day. This data was analyzed using a multiple comparison Dunnett T3 test because this data was not homogenous. This study was done in Pharmacological Laboratory Universitas Padjadjaran from October toNovember 2012. Results: The average of creatinine levels was as follows 0.62, 0.87, 0.98, and 0.87 mg/dL observed from the different groups. It was statistically shown that the significantly different creatinine levels, differing by 0.038 (p<0.05) were observed for the control group and the group which was given an infusion of breadfruit leaves. The significant difference on the average of creatinine levels were among the groups with (p-value <0.05).Comparison of group 1 and 3 (p < 0.05) gave a significant increase among the groups. Conclusions: Infusions of breadfruit leaves have some effects on blood creatinine levels in acute renal failure according to the dosage and duration of administration. [AMJ.2015;2(4):