Background and Aim: Neobenedenia girellae and Haliotrema epinepheli are important but neglected helminth parasites of marine fish. The humpback grouper, the most relevant definitive host, harbors several Neobenedenia and Haliotrema spp. simultaneously on body surfaces and gills. These species can be distinguished morphologically This study aimed to identify Neobenedenia and Haliotrema spp. infestations in monogenean humpback grouper by multiplex polymerase chain reaction method, which seems to be widely distributed in the study area. Data can be used as a basis for mapping disease patterns in Strait waters. Materials and Methods: Eighty humpback groupers (Cromileptes altivelis) were collected from eight different areas in the Sunda Strait and examined using scrapings from body surfaces and gill lamellae followed by multiplex PCR for identification. Results: Parasites on body surfaces were recovered from 49 fish (61.2%) and were found on gill lamellae in 72 fish (90%) by microscopic examination. Endoparasites were absent. Ectoparasites identified included, N. girellae, Neobenedenia melleni eggs, Neobenedenia pasifica, Neobenedenia longiprostata, Haliotrema eukurodai, H. kurodai, Haliotrema leporinus, Haliotrema dongshaense, Haliotrema angelopterum, Haliotrema aurigae, Haliotrema scyphovagina, and H. epinepheli. Conclusion: The distribution of trematode disease in humpback grouper in Sunda Strait waters was revealed. All parasites were from genera, Neobenedenia and Haliotrema. Risks associated with these parasites should not be overlooked. Prevention and control programs need to be extended to other marine fish. Humpback grouper should be dewormed more frequently.

Barramundi (Lates calcarifer) is a fish that has high economic value and has the potential to be developed in Indonesia. One of the species reported to barramundi is infest Neobenedenia girellae. This study aims to determine the prevalence and intensity of the parasitic worm Neobenedenia girellae in barramundi in the waters of Lampung and Situbondo. Sampling of barramundi with a size of 21 - 30 cm as many as 40 fish from Lampung waters and 50 barramundi from Situbondo waters. The results showed that there was worm infestation Neobenedenia girellae on barramundi in the waters of Lampung and Situbondo. The prevalence value of Neobenedenia girellae in barramundi in Lampung waters is 92% with almost always category and intensity value of 1.37 with low category when in Situbondo waters the prevalence value is 92% with almost always category and the intensity value is 1.67 with low category. Keywords: Lates calcarifer, Intensity, Prevalence, Neobenedenia girellae

Barramundi (Lates calcarifer) fish is one of the fishery commodities in Indonesia which has economic value and is the most exported commodity. The problem that often occurs in the process of cultivating barramundi (Lates calcarifer) is a parasite from the Haliotrema sp. which attacks the gills and can cause a decrease in the level of fish production. One way to determine the parasite infesting barramundi (Lates calcarifer) is by conducting a molecular identification. This study aims to determine the parasites by molecular identification and prevalence rate of ectoparasite worms in the gills of barramundi (Lates calcalifer) fish in Lampung waters. A sample of 40 fish with a length of 25-35 cm was taken by using a purposive sampling method. The organs observed were fish gills, then molecular identification was carried out by using real-time PCR, and the prevalence calculations. The results of this study indicated that the fish gills were infested by ectoparasitic worms, according to the NCBI GenBank database, called Haliotrema susanae with the band at 748 bp, and the prevalence rate of infested fish with gill’s ectoparasites is 80%.

Silver pompano (Trachinotus blochii) is one type of mari-culture that has high economic value and both national and international markets. The cultivation technique of silver pompano (T. blochii) used in floating net cages is inseparable from the common problems in every cultivation activity. one of these obstacles is the problem of infectious diseases, where is caused by disturbances this disease causes in fish metabolism by organisms such as parasites. The aim of this study was to identify the types of endoparasite worms based on molecular and the prevalence of endoparasite worms in silver pompano in floating net cages of Mari-culture Center, Lampung. It was done by taking 45 samples of fish with a length of 15-25 cm using a purposive sampling method. The observed internal organ of fish, then molecular identification, was carried out with conventional PCR and prevalence calculation. This study showed there was Camallanus carangid by DNA amplification fragments with a band of 972 bp and the prevalence value of 8.8%.