Abstract-Cadmium (Cd), a common toxic heavy metal, is widely distributed in the environment due to its use in industry. In the industry, Cd is hazardous both by inhalation and ingestion and can cause acute and chronic toxicity. Exposure to Cd had been known to cause damage to organs such as kidney, liver and testis. In this present study Cd was proposed caused an oxidative stress in ovarian rat (Rattus novergicus) cells. Female Rattus novergicus was divided in two groups, one control groups and one case group were exposed by Cd. Furthermore we set Superoxidismutase (SOD), Peroxidase (POX) and catalase (CAT) activity with Hydrogen peroxide (H 2 O 2 ) and Malondyaldehide (MDA) as a biomarker of oxidative stress. For analyzing of the data, SPSS software version 17 was used and was examined by Mean-Whitney test. For all outcomes, a nominal p-value of p<0.05 was considered significant. The resulted showed that there are a significance differences SOD, GPx and catalase activity between case and control group. The resulted also showed a significance differences H 2 O 2 and MDA levels between case and control group. The result suggest that Cd induced oxidative stress in ovarian cells.Index Terms-Antioxidant enzym, cadmium, ovarian cells and oxidative stress.