<p class="ISI-Paragraf">Jabodetabek and Bandung Raya metropolitan region experienced an urban expansion phenomenon that caused the two metropolitan regions to become increasingly connected by a corridor and form a mega-urban region caused by the conurbation process. Purwakarta regency is one of the regions in Jakarta-Bandung corridor that experienced the impact of Jakarta-Bandung conurbation process. This study aims to analyze the level of regional development, to analyze land cover change that occurred, and to predict Purwakarta Regency land use/land cover in 2030. Regional development analysis is done by using the Scalogram method based on Potential Village data of year 2003 and 2014. Land cover change analysis is done through spatial analysis by overlaying land cover Landsat Satellite Image of year 2000 and 2015. Land use/land cover prediction in 2030 is conducted through spatial modelling of Cellular Automata Markov method. Purwakarta Regency experienced an increase in regional development within the period of 11 years (2003 to 2014), which is marked by a decrease in the percentage of the number of villages that are in hierarchy III and increase in the percentage of the number of villages that are in hierarchy II and I. In general, within 15 years (2000 to 2015) Purwakarta Regency has increasing number of built-up area and mixed gardens, meanwhile dry land, forest, paddy field, and water bodies tend to decrease. The results of CA Markov analysis show that the built-up area is predicted to continue to increase from 2000 to 2030, meanwhile paddy fields and water bodies will continue to decrease.</p>

The distribution and composition of extracellular matrix (ECM) of the spleen in two species of fruit bats, namely Cynopterus titthaecheilus and Rousettus leschenaultii, were examined by histochemistry and immunohistochemistry. Reticular fibres accompanied by laminin were identified to make up the splenic stromal network. Types I and III collagen were identified in various spleen compartments with varying intensities. Thin and short elastin fibres were scattered in several parts of the spleen. Visualization of the ECM of the spleen can better demonstrate spleen compartmentalization. The alleged vascular space structure in the fruit bats spleen was the sinus structure that was strengthened by the presence of reticular fibres that limit the sinus basement membrane. The present study identified periellipsoidal lymphoid sheath (PELS)‐like structure in fruit bats spleen that had never been identified in mammals before. In addition to describing the structure, this study highlighted the variations in ECM composition of the spleen between species that can provide new insight into the phylogenetic study of spleen morphology.

The conventional anatomical study of specimens requires cutting processes which destruct the limited specimens. A non-destructive method, namely an ultrasonography, can be used to assess the anatomical organ information of those specimens. The aim of this research is to analyse the macroanatomy of the female reproductive organ in the Sunda porcupine (Hystrix javanica), using ultrasonographical imaging. In this study, four formaldehyde-fixed reproductive organ specimens of the Sunda porcupine were used. A 10-12 MHz linear ultrasound transducer was utilized to provide an imaging format of both longitudinal and transversal views. Photographic images were then used as comparison with a sonographic image. The results show that the ultrasound image of the Sunda porcupine reproductive organ soft tissue was hypoechoic, the lumen and antrum follicles were anechoic, while atretic follicles and the mons pubis were hyperechoic. Generally, the size of the organ was not significantly different between photographical and ultrasonographical imaging (p > .05). In conclusion, ultrasound images can be utilized for anatomical studies of the Sunda porcupine reproductive organs without destructing the specimen.

Landak jawa (Hystrix javanica) memiliki keunikan pada rambutnya yang termodifikasi menjadi duri. Duri pada landak berperan sebagai pertahanan diri dari predator. Penanganan dan pengekangan landak cukup sulit dilakukan sehingga pengambilan data biologi ladak jawa menjadi terbatas. Penanganan dan pengekangan secara kimiawi menggunakan sediaan bius umum digunakan pada hewan liar. Studi ini menggambarkan metode penanganan dan pengekangan fisik untuk pengambilan contoh biologi secara noninvasif pada landak jawa (H. javanica). Penggunaan kandang jepit yang dimodifikasi dengan penambahan sekat bergerak berbahan logam pada bagian dalam efektif untuk penanganan dan pengekangan landak jawa. Data biologi dari landak jawa yang dapat diambil untuk menilai status reproduksi dengan metode tersebut meliputi gambaran sel epitel vagina dan suhu tubuh.

Abstract. Yuliastuti, Prawira AY, Wahid ME, Nisa’ C, Agungpriyono S. 2022. Morphological investigation of intestine structure of the Sunda Porcupine (Hystrix javanica). Biodiversitas 23: 4793-4799. The intestine is an important part of the digestive system, which plays a role in the enzymatic process of food and the absorption of nutrients for survival. Sunda porcupine is one of the wild and protected animals in Indonesia and belongs to the group of rodents that have hindgut fermenter characteristics. Therefore, this study aims to investigate the intestine structure of the Sunda porcupine as an important part of the digestive system. Three adults of Sunda porcupine were euthanized in accordance with ethical approval of the Animal Care and Use Committee (ACUC) of IPB University (No. 92-2018 IPB). Gross anatomy of the macrostructure and length of the intestine, as well as histology features, such as general structure and carbohydrate content using PAS and AB pH 2.5 staining method, were observed. The organ consisted of the small and large intestine, with the duodenal ampulla and large caecum as the prominent structure. In this study, body weight and length correlated positively to intestinal length. The histological observation showed the presence of Brunner’s gland in the duodenum and it has the highest villi compared to others, while villi was absent in the large intestine. Furthermore, the small intestine exhibited various intensities with PAS and AB stain, while the large intestine had strong intensity in the goblet cell. Brunner gland in duodenum demonstrated the moderate intensity of PAS stain compared to the goblet cell in the mucosa. This study provides information about the intestine structure of Sunda porcupine as the herbivore and hindgut fermenter