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, T. 2010. Identification, sequencing and characterization of a stress induced homologue of fructose bisphosphate aldolase from cotton. Can. J. Plant Sci. 90: 41Á48. A drought-induced homologue of fructose-1, 6 bisphosphate aldolase full length cDNA (GaAldp) was isolated and sequenced by gene homology and rapid amplification of cDNA ends (RACE) from cotton variety FDH-786 (Gossypium arboretum). Quantitative polymerase chain reaction (PCR) analysis showed that drought stress, salinity and exogenous treatment with abscisic acid (ABA) enhanced the accumulation of GaAldp transcripts in the leaf and stem tissues of the plant. An alignment of the 1413 bp cDNA and 1937 bp genomic DNA sequences revealed that GaAldp has an open reading frame (ORF) encoding 394 amino acids and contains five introns. The predicted amino acid sequence is homologous to a heat-induced isoform of oat chlorplastic fructose bisphosphate aldolase (FBP) (AF216582) and NPALDP1 from Nicotiana paniculata. The GaAldp sequence includes a novel stroma targeting N-terminal transit peptide (TP) of 45 amino acids, which is 63Á76% similar to other chloroplastic TPs.

Background Sand flies are of considerable public health importance in Pakistan because these insects are vectors of leishmaniasis. The current study explores the bionomics of sand flies, their spatial distribution pattern and cutaneous leishmaniasis-associated risk factors in District Mohmand, Khyber Pakhtunkhwa, Pakistan. Methods Sand flies were collected from indoor and outdoor habitats in 69 villages of five tehsils in Mohmand during July–October 2019. Risk factor data were recorded for 829 households in 94 villages. Results In total, 2065 sand flies were captured. Phlebotomus (Paraphlebotbmus) sergenti was the most abundant species. Relative density for P. sergenti and Phlebotomus papatasi was highest in Prang Ghar and lowest in Safi. Sand flies abundance peaked in August and September, corresponding to maximum relative humidity, temperature and rainfall. Relative density for P. sergenti and P. papatasi was highest in combined dwellings (indoor) and cattle corrals (outdoor). Phlebotomus sergenti and P. papatasi were abundant at an elevation of 283–1140 m on agricultural land and rangelands. Both species were recorded abundantly on Carbontites, Mesozoic and Indus suture Melange rock formations. Presence of domestic animals, ownership of pet dogs, presence of muddy dunes in the village, knowledge of sandflies and use of mosquitoes spray remained significant risk factors. Conclusions The study reports sand fly bionomics in District Mohmand. Risk identified for cutaneous leishmaniasis are significant in strategising control methods for Health authorities can allocate localized control means to high-risk areas using these findings.

The recent study enlightens the synthesis and characterization of zirconia (ZrO 2 ), Fe-doped ZrO 2 (Fe@ZrO 2 ), and Ni-doped ZrO 2 (Ni@ZrO 2 ) catalysts having new-fangled morphology with tuned band gap as photocatalysts for degradation of textile wastewater dyes methylene blue (MB), malachite green (MG), and their mixtures. SEM imaging of Fe@ZrO 2 and Ni@ZrO 2 is interestingly varied from ZrO 2 as well as doping of transition metals greatly affects the morphology of composites. The optimization study depicts that the pH, time (min) and catalyst amount (g) have a direct relation with degradation efficiency, while the dye concentration (mg/L) has an indirect relation as well. The photocatalytic studies depict that the degradation of MB and MG follows oxidation pathway via hydroxyl radicals (OH • ) and holes. Reusability of catalysts corresponds to a little decrease in degradation efficiency in the first two cycles and decreases to about $10% (Ni@ZrO 2 ) and $12% (Fe@ZrO 2 ) in next three cycles.

Aim and Objective: This study aims to determine the effectiveness of laparoscopic appendectomy for children performed as emergency or emergency surgery at MTI/LRH in Peshawar, Pakistan. Material & Methods: From January 2020 to December 2022, the Department of Pediatric Surgery, Medical Teaching Institute, Lady Reading Hospital Peshawar studied acute appendicitis in children. This study comprised children with clinically confirmed acute appendicitis as indicated by blood complete, HBs Ag, HCV, ultrasound, and primary assessment. The pediatric surgical unit maintained direct resuscitation of patients' NPO for at least 6 hours, began antibiotics, and performed laparoscopic surgery under general anesthesia. This strategy avoids additional treatment, saves time and money, reduces hospital-acquired infections and other hospital dangers, and allows for excellent local infection control without pus spreading throughout the peritoneal cavity. Laparoscopic surgery makes cholecystectomy a few-day procedure instead of a week. Laparoscopic appendectomy reduces hospital stays for children. Thus, we examined our data to determine whether children may have an emergency or same-day laparoscopic appendectomy (24-hour postoperative stay). We reviewed all three-year-olds who underwent laparoscopic appendectomy for suspected appendicitis. Results: Acute appendicitis afflicted 79 children, 4/1 male-to-female. 79 2- to 16-year-olds underwent laparoscopic appendectomy surgeries. 5% of children after laparoscopic appendectomy developed perforated appendicitis and needed an open appendectomy. Forty-one (64.5%) had severe appendicitis before surgery. 22 (27.2%) had perforated appendicitis, (5%) had ovarian cyst rupture, and 2.5% had no pathology. Surgery averaged 54 minutes. 14 of 79 primary wound closure patients had their right lower quadrant peritoneal cavity emptied. All 79 patients underwent successful appendectomy operations. Wound infection (6 patients) and stomach pain (4 patients) were the most prevalent sequelae managed conservatively. Four patients required readmission and laparotomy due to intestinal obstruction and other issues. Thirty-two children were released within 24 hours after laparoscopic appendectomy. The control group had no significant morbidity (drug rash,1: fever, 3). Practical implication: This study will provide the data on the effectiveness of the laparoscopic appendectomy for the treatment of Appendicitis. Conclusion: Our study shows that the current laparoscopic appendectomy method for treating adorable appendicitis in children is safe and may be performed as a fast-track or same-day operation with a postoperative stay of fewer than 24 hours in some children who do not have perforated appendicitis. Keywords: Laparoscopic appendectomy, Pediatric Laparoscopy, fast-track same-day surgery