Abstact. In this study, the effect of “Zamin-M” biopreparation on the content of some macronutrients, microelements and flavonoids in the leaf extract of thorny artichoke (Cynara scolymus L) belonging to the family Asteraceae was determined by 4 different factors: control, mineral fertilizer, biopreparation, biopreparation study and mineral fertilizer. The order of decreasing the concentration of macro-microelements in the control variant changed for K>Na> Ca>Mg>P and Fe>Si>Al>Mn>Zn>Cu>Se>Co. In plants treated with “Zamin-M” biopreparation, changes in Ca>Na>Mg>K>P and Fe>Al>Si >Mn>Zn>Cu>Co>Se were found. When determining the effect of Zamin-M biopreparation on the flavonoids in artichoke leaves, it was noted that the amount of quercetin in plants increased by 93.05% compared to plants treated with mineral fertilizers. The amount of rutin was increased by 54.94% in plants treated with “Zamin-M”, it was 57.55% in plants treated with mineral fertilizers, and it was 59.60% in plants treated with mineral fertilizers + “Zamin-M” biopreparation.