In the present study the crude oil and defatted seed cake were analyzed to calculate phenolics, flavonoid, totaltocopherol and DPPH activity for two locations. The C. fistula had highest phenolics 12.0±0.0 mgGAE/g, flavonoids content 2.9±0.0 mgCAE/g, total tocopherol 185.6 mg/g and carotenoids was 131.0 mg/kg. The maximum antioxidant activity was 83% and in terms of IC 50 value was 0.041±0.0 mg/ml. The fatty acids profile shown that the significant amount of fatty acids were linoleic and oleic acid.

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The antioxidative effect of acetone extracts of sesame and sunflower meal on stability of crude and refined soybean oil (C&RSO) stored for 120 days at 50 ºC was determined by monitoring the free fatty acid, p-anisidine value and peroxide value. The oil was dosed with different concentrations (500, 1000 and 2000 ppm) of the extracts and tert-butylated hydroxyquinone and propyl gallate (conc. of both 200 ppm). Free fatty acid, p-anisidine value and peroxide value of crude and refined soybean oil containing sesame and sunflower meal extract were lower than crude and refined soybean oil containing no additive. However, refined soybean oil had much higher free fatty acid, p-anisidine value and peroxide value than crude soybean oil because some natural antioxidants such as carotene, tocopherol lost during refining. The sesame and sunflower extract have better antioxidant activity than propyl gallate but less than tert-butylated hydroxyquinone. Results suggest that sesame and sunflower meal extract could be used as alternative natural antioxidants with wide food applications.