In the dark, the pineal gland secreted melatonin hormone after produced from tryptophan that is Responsible for organizing many vital functions such as Wake up and sleep, circadian rhythm, Immune response and reproduction. As well as that Melatonin act as antioxidant and inflammatory. Thirty-six of 45 days-old Japanese quail male randomly divided to four treatments nine replicates per each. The control treatment consisted of the basal diet and normal water, 2nd treatment of (1%) hydrogen peroxide in drinking water.3rd and 4th treatment the ratio supplemented with (20 mg melatonin / kg diet) with or without(1%) hydrogen peroxide in drinking water. At 28 days, hematological parameters, antioxidant measurement determined and testes histological assessment. From the results noted a significant reduction in the total counting of red blood cells (RBC) and the relative weight of the testicles with a deterioration in antioxidants status, represented by a significant increase in MDA level and a significant decrease in GSH level of with H2O2 treatment . The supplemented of melatonin led to a decrease in the total number of WBC, PCV, and the concentration of Hb. Moreover, the treatment with the use of melatonin led to an improvement in antioxidants indicators represented by an increase in the level of GSH and a decrease MDA level. As the fourth treatment did not record significant differences compared to the first treatment. Adding melatonin at 20 mg melatonin / kg diet lead to significant improvement in area of germinal layer and its thickness.