Rising petroleum costs, trade imbalances and environmental concerns have stimulated efforts to an advance in the microbial production of fuels from lignocellulosic biomass. Ethanol, the most widely used renewable liquid transportation fuel, has only 70 % of the energy content of gasoline and its hygroscopicity makes it incompatible with existing fuel, storage and distribution infrastructure. Saccharomyces cerevisiae is the only yeast that can rapidly grow under aerobic as well as in anaerobic conditions. This unique ability plays a major role in various industrial applications of S. cerevisiae, including beer fermentation, wine fermentation and large-scale production of biofuel ethanol. Here we focused on Induced Mutations for Yeast to attain better Bioethanol yield from Fruit waste. We planned for other substrates such as the fruit waste example banana peel, chikko peel, mango peel, pine apple peel and other fruit waste. Ethanol estimated by chromic acid assay, Induction of mutation by EMS &UV. Ethanol estimated by Gas chromatography, Isolation of genomic DNA using mini prep filters. The Results obtained from the Gas Chromatography for the Saccharomyces cerevisiae-EMS & UV Mutated, the Purity level for the Ethanol was found to be 73.5 % & 53.9 % respectively, with acid treatment it showed 53.9 %.The Genomic DNA bands of S. cerevisiae (control) and mutated (UV & EMS) has shown slight variation since, it could be responsible for the higher ethanol production, this method can be utilized for renewable source of energy & domestic wastes with sustainable technology.