MethodsMice. C57BL/6 and BALB/c mice were obtained from Harlan (Zeist, the Netherlands). C57BL/6.CH-2 bm12 (bm12) mice were obtained from The Jackson Laboratory (Bar Harbor, Maine, USA). C57BL/6 IL-5 knockout mice (23) were kindly provided by M. Kopf (Basel Institute for Immunology, Basel, Switzerland).Antibodies. The hamster anti-mouse CD3 mAb 145-2C11, the IgG1 rat anti-mouse IL-5 mAb TRFK-5, the IgG1 rat antimouse IL-4 mAb 11B11, and the IgG1 rat anti-mouse IL-10

Eosinophil infiltrates, typical of MHC class II disparate acute skin graft rejection, are critically dependent on the availability of IL-4. IL-4 mice reject MHC class II disparate skin grafts by a pathway of rejection where neutrophils play a direct causal role.

No abstract