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Production of compounds of Pharmacological importance from earthworms is a novel area that has gained importance in many of the South East Asian countries in modern day medicine termed as vermiceuticals. Earthworms were maintained in plastic tubs containing decomposed organic matter that served as substrate and their feed. Cancer cell were cultured according to standard cells culture method. The cancer cells were treated with different concentrations of proteins from coelomic fluid of Eudrilus eugeniae to study the concentration effect on cytotoxicity using MTT assay. The result revealed that the coelomic fluid of Eudrilus eugeniae induced cell death in 48hrs and the activity was concentration dependent. In the present study Cytotoxic effect of coelomic fluid from Eudrilus eugeniae was studied on HeLa cell, Colon cancer cells, WBC malignant tumor cells and Brain tumor cells. This confirms the presence of cytotoxic molecules in the coelomic fluid of earthworms. This work suggests that some of the coelomic fluid components might be useful for pharmaceutical applications in the treatment of cancer.

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The current study indicates that octyl-β-D-glucopyranoside (OGP) as a detergent which has the ability to make the lipid layer stiff. OGP was subjected for toxicity studies and in vitro cytotoxicty assays on cancerous HeLa and non-cancerous myoblasts H9c2 cell lines. Test against aquatic organisms were carried out in Artemia salina and LC values were calculated. Hemolytic activity tested for blood bio-compalibity showed hemolysis rate of 10-16%, followed by thrombolytic activity to burst the clots in blood. Also, the samples showed good lysis when compared to the standard streptokinase. Furthermore, α-amylase activity has been carried out to check the inhibition of α-amylase by the OGP. Finally, antibacterial activity has been tested against four different pathogens and their MIC values have been calculated.

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