This study enlightens the methods used for the identification as well as the growth of Spirulina species in Pakistan. Spirulina was identified based on morphology using microscopic technique along with its biochemical identification using SDS PAGE. After the endorsement of spirulina species via microscopy and SDS PAGE, Spirulina was subjected to growth in Zarrouk’s media. Efficacy of growth was observed using three different methods Tubular photobioreactors, Fed-batch photobioreactors, and Attached cultivation which boosts the yield of Spirulina biomass via improving its biochemical composition. Hence, it concludes that production of the Spirulina using tubular photobioreactors on pilot-scale yields cost-effective benefits and provides us with an enhanced biochemical content ratio of Spirulina and can easily be identified by using several approaches including biochemical, molecular, and microscopic methods.