This study was conducted to examine the effect of adding different concentrations of nano silver nitrate (0, 5, 10 and 15 mg L-1) and zinc source {traditional(0.86mg L-1 as ZnSO4.7H2O) and nano zinc  0.086mg L-1 as Zinc oxide) to the specifies medium of rooting strawberry (Fragaria X ananassa Duch) cv. Rubygem, in addition to testing the efficiency of nano silver nitrate and zinc source on successful percentage of cultures adapting stage. Results revealed that highest rooting percentage, root numbers, roots length, leaves content of total chlorophyll and macro nutrients (N, P and K) were achieved the highest average at 10mg L-1 + nano zinc treatment. The source of cultures resulting from rooting stage affected the plant survival at acclimatization stage. The treatment 10 and 15mg L-1 of nano silver nitrate + nano zinc achieved 100% success percentage.

Strawberry (Fragaria ananassa Duch), belong to Rosaceae family, is widely appreciated, mainly for its fruit characteristic aroma, bright red color, juicy texture, It is traditionally asexually propagated by rooted runners. To improve the strawberry varieties this method was not suitable due to incidence of many serious diseases infection resulting in the gradual degeneration of cultivars performance, so micropropagated strawberry plant has been introduced to prevent most of the plant and soil transmissible diseases. Micropropagated strawberry has several advantages, such as its ability to multiply virus free stock rapidly and, the improved capacity of these plants to produce runners for planting in the field. In this study, it was aimed to examine the interaction of BA, IBA and AgNO 3 combinations at different micropropagation stages. Results showed the addition of AgNO 3 to MS medium led to increase shoot number, shoot length, fresh and dry weight at multiplication shoots stage, shoots rooting % and root number at rooting stage. Survival plants %, plant height, leaves number and dry weight at acclimatization stage.