With development of nanotechnology, the biological synthesis process deals with the synthesis, characterization, and manipulation of materials and further development at nanoscale which is the most cost-effective and eco-friendly and rapid synthesis process as compared to physical and chemical process. In this research silver nanoparticles (AgNPs) were synthesized from silver nitrate (AgNO 3 ) aqueous solution through eco-friendly plant leaf broth of Ocimum sanctum as reactant as well as capping agent and stabilizer. The formation of AgNPs was monitored by ultraviolet-visible spectrometer (UV-vis) and Fourier transform infrared (FTIR) spectroscopy. X-ray diffraction (XRD) and scanning electronic microscopy (SEM) have been used to characterize the morphology of prepared AgNPs. The peaks in XRD pattern are in good agreement with that of face-centered-cubic (FCC) form of metallic silver. Thermal gravimetric analysis/differential thermal analysis (TGA/DTA) results confirmed the weight loss and the exothermic reaction due to desorption of chemisorbed water. The average grain size of silver nanoparticles is found to be 29 nm. The FTIR results indicated that the leaf broths containing the carboxyl, hydroxyl, and amine groups are mainly involved in fabrication of silver AgNPs and proteins, which have amine groups responsible for stabilizing AgNPs in the solution.

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