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In this study, biodiesel is produced from waste sunflower oil and its ef-fect on engine performance and specific fuel consumption is experimen-tally investigated. Transesterification method was used in biodiesel pro-duction of waste sunflower oil. After production, analyzes giving charac-teristics of biodiesel such as density, kinematic viscosity, sulfur and water content were made. As a result of the analysis, the biodiesel fuel with a viscosity of 4,372 mm2/s, which has a reaction temperature of 60°C, an alcohol content of 25% and a catalyst ratio of 0.05%, was cho-sen as a reference fuel, as it was observed to comply with European Standards of EN 14214. Standard diesel fuel was taken as reference for the purpose of examining the effect of biodiesel on engine performance. Performance and fuel consumption tests of the produced biodiesel and standard diesel fuel were carried out at the full load position at seven dif-ferent engine speed levels. Experiments were carried out on a single cy-linder four-stroke Cussons P8160 engine. As a result of the test, it was observed that there was a 7.06% reduction in engine moment compared to the standard diesel fuel use of B100 fuel. In addition, compared to the standard diesel fuel of B100 fuel, there was a 6.64% reduction in engine power. Specific fuel consumption values for B100 and diesel fuels at 2200 rpm engine speed were 426.75 g/kWh and 355.22 g/kWh, respecti-vely.

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