Repeated crystallization or the use of different chemicals to obtain a pure crystal can cause yield/purity issues. Therefore, impurity penetrating the crystalline mesh should be removed as much as possible...

Saf bir kristal ürün elde etmek için tekrar tekrar uygulanan kristalizasyon basamakları veya farklı kimyasallara başvurulması, bitmiş ürünün veriminde farklı sorunlara neden olabilir. Bundan dolayı, kristal ağın içerisindeki safsızlıklar, ana ürünü etkilemeden uzaklaştırılmalıdır. Bu çalışmada, Amoksisilin trihidrat (Amct) ve safsızlık olarak 4-hidroksifenilglisin (4-HPG) kullanılmıştır. Amct kristal kafesinden, safsızlık olarak 4-HPG’nin yıkama yöntemleri kullanılarak uzaklaştırılması gösterilmiştir. Ayrıca, bu çalışmada ilk kez, yıkama verimi ile yıkama yöntemleri, çözeltiler ve sıcaklıklar arasındaki ilişkiyi incelemek için Taguchi metodu kullanılmıştır. Denemelerde bir Büchner cam hunisi istenilen sıcaklıkta ve ürün kaybı olmadan çalışılacak şekilde yeniden tasarlanmıştır ve denemeler burada yapılmıştır. Bu çalışma, farklı yıkama yöntemleri, kullanılan Taguchi metot ve yeniden tasarlanan Büchner cam hunisi ile literatüre farklılık katmaktadır. Etki grafiklerinden yıkama verim değerlerini etkileyen faktörler sırasıyla yıkama yöntemi, sıcaklık ve çözelti olarak belirlenmiştir. En yüksek verim, tek aşamalı yıkama yöntemi ile Etilalkol kullanılarak 15°C’de elde edilmiştir.

Amoxicillin is one of the broad-spectrum β-lactam antibiotics widely used in the treatment of many diseases. It is inevitable that 4-hydroxyphenylglycine (4-HPG) and 6-Aminopenicylanic acid (6-APA), which are used during the production of this antibiotic, are incorporated into the molecular lattice of the product as impurities. Today, many expensive methods and chemical devices are used for the purification of Amoxicillin by determining 6-APA and 4-HPG, which are defined as impurities. In this study, it was aimed to develop a fast, simple, and specific UV-spectrophotometric method for the determination of 4-HPG and 6-APA. Another aim of this article is to cause as little harm as possible to the environment and human health by using as few chemicals as possible throughout the study. In this study, all attempts to determine 6-APA and 4-HPG, which are impurities in the production of amoxicillin, were carried out with the help of a UV/VIS spectrophotometer. Also, Four different concentrations of NaOH were used as a solvent for each impurity. UV spectra of 4-HPG and 6-APA concentrations between 210 and 400 nm were measured. In the literature, the UV spectrum of 4-HPG has been revealed for the first time in this study and examined in detail. The UV spectrum of 4-HPG was characterized in 3 regions. Again, the response of 6-APA to different NaOH concentrations was demonstrated for the first time in this study. It was determined that the peaks of 6-APA dissolved in NaOH shifted from 222 nm to 227 nm depending on the concentration amount. In addition, it is an ideal green procedure that makes a difference in the literature, as the study is carried out for the control and determination of impurities without the use of any organic solvents or chemicals harmful to the environment.

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