No abstract

No abstract

No abstract

No abstract

The present study was conducted to examine the protective roles of trolox and alpha-lipoic on post-thawed Pirlak ram sperm parameters, oxidative stress and DNA damage in non-breeding season. Semen samples from 5 healty Pırlak rams (2-3 years of age) were used in the study. Six ejaculates for each rams were collected and pooled. Ejaculates were split into three aliquots and diluted to a final concentration of 150x10 6 spermatozoa/ml with the base extender containing 1 mM alpha lipoic acid, 1 mM trolox and no additive (control) were cooled to 5 °C and equilibrated for 3 h then frozen in 0.25 ml French straws. Samples were thawed in 37 o C and evaluated in terms of spermatological parameters, DNA damage, oxidative stress. Sperm motility was increased, acrosome rate and DNA damage were decreased significantly (p<0.05) in ALA, head abnormal sperm rate and membrane integrity were increased, acrosome rate and DNA damege were decreased significantly (P < 0.05) in trolox when compared to the control group. Compared with the control group, reductions in spermatozoon MDA and GSH levels were statistically significant (P <0.05) in lipoic acid and trolox groups. Results of this study suggest that alphalipoic acid and trolox improve sperm parmeters, oxidative stress and DNA damage in non-breeding season.