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Growth promoting and immune stimulating effects of common mallow (Malva sylvestris) aqueous methanolic extract (AME) in O. mykiss were examined. Two different concentrations of common mallow AME [0.1 (CM1) and 0.5 (CM5) g kg -1 of feed] commixed a basic diet and a control diet without the common mallow extract were fed to rainbow trout for 30 days to evaluate growth rate and immune responses. At the end of the study, fish growth performance was determined as significantly higher in the group fed with 0.1 g kg -1 common mallow AME compared with control diet fed group (P<0.05). In fish fed with CM5 diet, oxidative radical production (ORP) was the highest (P<0.05). Similarly, myeloperoxidase (MPO) activity was increased significantly in CM5 group. Lysozyme (LYS) and phagocytic activities (PA) were not altered in treated fish groups compared to the control group (P<0.05). The pro-inflammatory (IL-1β, TNF-α1, IL-8,), anti-infammatory (IL-10), lymphocyte agonist (TGF-β) and cell-mediated immune regulatory, IL-12 cytokines were generally down-regulated insignificantly compared to control. Although, common mallow AME could not elicit cytokine-mediated immunity and resistance to bacterial pathogen, A. hydrophila in rainbow trout, elevation in ORP and MPO activities in treated groups and growth promoting effect were noticed at a low dose. Therefore, application AME of common mallow at 0.1 g kg -1 of feed as growth promoter and non-specific immunostimulant is advocated for rainbow trout.

In the present study, a 150 cubic m net pen was designed as part of a collaborative research effort between the International Copper Association (ICA-USA), the University of New Hampshire (UNH-USA) and Cannakale Onsekiz Mart University (COMU-Turkey) in August 2011. The fish cage was developed to support the creation of a small scale demonstration farm, located in the Strait of Canakkale, off the coast of Guzelyali town in Turkey. The surface gravity-type, octagonal shaped fish cage was designed to have a diameter of 6 m and a copper alloy mesh chamber depth of 5 m. The present study details the cage construction and system deployment of one fish cage utilized a chain link mesh net chamber with a copper alloy developed by Wieland-Werke in Germany, with reference to work load challenge and estimation of man-power necessary for the partial and total work efforts. As a conclusion, one cage equipped with copper-alloy mesh pen was brought to a final shape with the net chamber assembled and attached to the cage frame in 3 days and 90 man-hours. The HDPE (high density polythylene) cage frame was assembled by an outside company, therefore detail of the main cage frame is not discussed in this paper.