This study aims to find out the effectiveness of ethanol extract rhizome curcuma (Curcuma xanthorrhiza Roxb.) as an antipyretic in mice(Mus muscullus L.) hyperpireksia. This study is an experimental study with the Complete Randomized Design (RAL) method. A total of 15 male mice weighing 30-35 grams were divided into 5 treatment groups namely KN (drinking water), KP (paracetamol), P1 (extract 3 mg/gr BB), P2 (extract 8 mg/gr BB) and P3 (extract 14 mg/gr BB). Hyperpyreksia is obtained by inducing mice with 10% peptons. When it reaches above normal temperatures (35.50C - 37.00C), it is given extracts according to the treatment group and measured its effect at minutes 30, 60 and 90. The data was analyzed using ANOVA with α=0.05 and advanced tests of LSD. The results showed P3 was more effective and faster at lowering body temperature, compared to KN, P1 and P2. Seen in the 90th minute the body temperature squeaked P3 (35.960C) back to normal temperatures and closer to KP (35,760C), after which followed by P2 (36.30C). KN (38,630C) and P1 (37,630C) are still categorized as high, as they have not returned to normal temperatures. The results can be concluded that the ethanol extract of curcuma rhizome has antipyretic activity. ABSTRAK Penelitian ini bertujuan untuk mengetahui efektivitas ekstrak etanol rimpang temulawak(Curcuma xanthorrhiza Roxb.) sebagai antipiretik pada mencitMus muscullus L.) hiperpireksia. Penelitian ini merupakan penelitian eksperimental dengan metode Rancangan Acak Lengkap (RAL). Sebanyak 15 ekor mencit jantan berat 30-35 gram dibagi dalam 5 kelompok perlakuan yaitu KN (air minum), KP (parasetamol), P1 (ekstrak 3 mg/gr BB), P2 (ekstrak 8 mg/gr BB) dan P3 (ekstrak 14 mg/gr BB). Mencit hiperpireksia diperoleh dengan cara menginduksi mencit dengan pepton 10 %. Ketika mencapai di atas suhu normal (35,50C - 37,00C), maka diberikan ekstrak sesuai kelompok perlakuan dan diukur pengaruhnya pada menit ke 30, 60 dan 90. Data dianalisis menggunakan ANOVA dengan α=0,05 dan uji lanjut LSD. Hasil penelitian menunjukkan P3 lebih efektif dan lebih cepat menurunkan suhu tubuh mencit, dibandingkan dengan KN, P1 dan P2. Dilihat pada menit ke-90 suhu tubuh mencit P3 (35,960C) kembali ke suhu normal dan lebih mendekati KP (35,760C), setelah itu diikuti P2 (36,30C). KN (38,630C) dan P1 (37,630C) masih dikategorikan tinggi, karena belum kembali ke suhu normal. Hasil tersebut dapat disimpulkan bahwa ekstrak etanol rimpang temulawak memiliki aktivitas antipiretik.Kata Kunci : Antipiretik, Temulawak, Mencit, Efektitivitas, Hiperpireksia.

A contact lens cleaner solution should not be left in place for storage and reuse for subsequent storage. The cleaner that has been used does not have enough disinfectant power, even becomes a place of microorganism growth so the risk of infection increases. The study aims to identify the bacterial isolates of the beta hemolysis contained in the residual cleaning fluid the lens contact. The sample in this research was taken from all students in the STIKES Mandala Waluya Kendari who wear contact lenses as many as 10 people. The suspension of bacteria obtained is then planted on media BA (Blood agar) with the streak plate method. Isolates showing beta hemolysis then identified using amplification gene 16S rRNA using primers universal primers 27 F/1495 R, the results of the amplification electrophoresis with agarose of 1.5% and subsequent sequencing on the gene 16S rRNA. Based on the results of this study show the 10 isolates,

This study aimed to determine the morphological characteristics of Phytophthora sp. isolated from cocoa fruits from Olo-oloho Village, Konawe Regency, Southeast Sulawesi. Isolation of Phytophthora sp. carried out by the point method using V4 (Vegetable Juice Agar) media incubated at 27ºC for 24 hours. Morphological characterization of Phytophthora sp. included characterization of colony morphology and cell morphology. The results showed that the colony morphological characteristics were white colonies, cotton-like textures, the uneven edge of the colony, zoning and radial lines. The morphological characteristics of the cell had asexual spores in the form of sporangium and chlamydospores, hyphae are not aseptic, greenish-black zoospores, zoospores are round and double-flagged, and have sporangiophores. Keywords: Phytophthora sp., colony morphology, cell morphology

Scad Mackerel (Decapterus spp.) is the main consumption commodity of coastal communities in Nambo village. Apart from public awareness is quite high, the price is affordable, and has nutritional value since unsaturated fats is low, so it good to consume for all ages. The public lack of knowledge about proper fish management. The solution is to provide an understanding of fish processing techniques so be more selling, delicious and nutritious, as well as for community empowerment with the aim of creating fisheries entrepreneurs. The method was the lecture method, socialization and training to the community as an empowerment effort to improve knowledge, skills and well-being. The implementation of the service in the form of location surveys, coordination with local regional chief of village and PKK administrators, preparation of equipment and materials, and some processed samples of fish, socialization about the benefits of fish and fish processing training

The purpose of this research is to know the character of partial sequences of the COI gene form fruit-sucking pest. The gene fragments were isolated using PCR (Polymerase Chain Reaction) techniques with specific primers, HlpF and HlpR. Character of gene fragments observed were fragment size, nucleotide sequence, similiarity, restriction map, and hydrophobicity. The size of the fragment was determined by electrophoresis of PCR products, similarity values were determined by aligning the nucleotide sequence of the PCR product with the nucleotides present in GenBank, the restriction map determined by the RestrictionMapper program, and the hydrophobicity profile determined by the BioEdit program. The results showed that PCR yield fragment size 552 pb. The results of alignment analysis showed that PCR fragment had similarity of 88% with Helopeltis theivora, 87% with Helopeltis antonii, 87% with Helopeltis bradyi and 84% with Pacipeltis maesarum. Based on the results of the analysis using RestrictionMapper program shows partial sequences of the COI gene form fruit-sucking pest has 25 sites of restriction enzyme cutting which is class of type II endonuclease enzyme. The results of the hydrophobicity analysis using the BioEdit program indicate that the COI protein is hydrophilic and hydrophobic which shows the integrated COI protein on the membrane.Keywords: COI gene fragment, fruit-sucking pest, PCR, Cocoa Crop