SummarySeven fractions obtained from wheat gluten by chromatography on carboxy~ methyl-cellulose were studied by ultracentrifugation, gel electrophoresis, chemical, and N-terminal amino acid analysis. On rechromatography, five fractions eluted by sodium chloride behaved as distinct entities. illtracentrifuge experiments indicated that four of these were each undergoing rapid, reversible association. Several N-terminal amino acids were found in each of the fractions which, moreover, could be resolved by the gel technique into a number of electrophoretic bands, some bands being common to those of neighbouring fractions. Total nitrogen values showed the chromatographic samples to be essentially free from non-protein material.

No abstract

SummaryThe use of an ultraviolet "peak-spotter" and pH-meter to obtain the first derivative of the effluent transmission curve from columns of Sephadex is described. The" method is of use in determining accurate peak separations in effluents, in handling cloudy solutions, as a guide to the fractionation of complex inixtures, and in the study of rapid reversible association in protein systems.