Sequences of the RNase P RNA gene were investigated for the phylogenetic analysis of the genus Saccharomonospora. Aligned nucleotide sequences, determined from the PCR-amplified RNase P RNA gene of representative strains of the genus Saccharomonospora, displayed 94.22103 O/ O interspecif ic variances. The intraspecific similarity value was 99.7-100 O/ O in all species tested. Saccharornonospora azurea K161 and 'Saccharomonospora caesia ' K76T displayed identical RNase P RNA gene sequences in the region that was determined and Saccharornonospora sp. K180 showed sequences distinct from validly described species with a similarity value of 946*1*0%. The phylogenetic trees constructed by aligning the sequences either within the genus Saccharomonospora or with other Gram-positive bacteria were similar to the ones derived using sequences of the 165 rDNA gene. Advantageous features of this gene for application as a molecular phyletic marker are discussed.

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