Fusion gene is an important class of therapeutic targets and prognostic markers in cancer. ChimerDB is a comprehensive database of fusion genes encompassing analysis of deep sequencing data and manual curations. In this update, the database coverage was enhanced considerably by adding two new modules of The Cancer Genome Atlas (TCGA) RNA-Seq analysis and PubMed abstract mining. ChimerDB 3.0 is composed of three modules of ChimerKB, ChimerPub and ChimerSeq. ChimerKB represents a knowledgebase including 1066 fusion genes with manual curation that were compiled from public resources of fusion genes with experimental evidences. ChimerPub includes 2767 fusion genes obtained from text mining of PubMed abstracts. ChimerSeq module is designed to archive the fusion candidates from deep sequencing data. Importantly, we have analyzed RNA-Seq data of the TCGA project covering 4569 patients in 23 cancer types using two reliable programs of FusionScan and TopHat-Fusion. The new user interface supports diverse search options and graphic representation of fusion gene structure. ChimerDB 3.0 is available at http://ercsb.ewha.ac.kr/fusiongene/.

In order to estimate the effective dose of mulberry leaf water extract (MLEs) and appropriate intake time, we carried out clinical trial for the suppression of postprandial blood glucose and measuring plasma 1‐deoxynojirimycin after consuming MLE. This trial was designed as a single blind, randomized, and parallel study. Fifty healthy volunteers (fasting blood glucose < 125 mg/dl) received 0, 1.25, 2.5, 5g of mulberry powder (0, 4.5, 9, 18mg of DNJ) followed by 75g of maltose and blood glucose levels were determined at 0, 30, 60, 120, 180 min after the MLE/maltose administration. Another group was ingested high dose MLE (5 g of MLE with 18 mg of DNJ) 30 min prior to the maltose administration, and then the blood glucose levels were also determined. Among the participants, the blood samples of 10 volunteers for high and low dose group were collected and analyzed plasma DNJ level. By MLE treatment, the blood glucose at 30min of 2.5 and 5g of mulberry powder groups significantly lower than the control group. Excursion and peak height of blood glucose also significantly decrease at the group of 2.5, 5g of mulberry powder. However, there is no significant difference at the group given 30 min before maltose administration. These results suggested that MLE show postprandial hypoglycemic effect on healthy subjects and established the basis of effective dose and time. This project was supported by the Ministry of Knowledge & Economy (RITD program, Project No. 70004683) and the Ministry of Education, Science and Technology (Brain Korea 21, Project No. 2006‐0519‐4‐7).