The purpose. To develop conditions of stimulation of latent buds on zingiber roots and method of growing aseptic culture in vitro. Methods. Laboratory and biotechnological. Results. Outcomes of probes on stimulation of buds on zingiber roots in conditions of thermal room and thermostat are brought. They compare characteristics of sterilizing solutions "Bilizna" and Sublimate of different concentrations and expositions against infection contamination of zingiber. Conclusions. For stimulation of buds on zingiber roots it is rational to use thermostat with temperature of 28±2°С and moistness of 90% during 1-2 months. At getting aseptic culture of buds of zingiber in vitro it is expedient to apply solution of sublimate with weight fraction of 0,1% and exposition of 45-55 minutes. That will allow to receive 61,4-81,9% of viable buds with sterilizing values 50,0-63,9%.