Vanda tricolor is an orchid endemic to Mount Merapi in Yogyakarta Indonesia, whose existence is threatened due to the eruption and exploitation of its original habitat. This orchid conservation effort can be done through in vitro culture. Multiplication is one of the in vitro culture techniques used for propagation of the Vanda tricolor orchid by growing explants on various types of multiplication medium. This study aims to determine the effect of medium types on the multiplication of Vanda tricolor orchids. The method used in this research is a laboratory research method prepared with a completely randomized design, with the treatment of the growth medium consisting of 5 types, namely New Dogashima Medium, Murashige and Skoog, Vacint and Went, leaf fertilizer and liquid organic fertilizer medium. All mediums were supplemented with 150 ml / l coconut water, 150 g / l banana extract, and 0.2 g / l activated charcoal. Each treatment was repeated 3 times, each replication consisted of 3 samples. The results showed that liquid organic fertilizer medium is the best medium for multiplication of Vanda tricolor orchids as indicated by the addition of the shoots.