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Electrocardiogram is a non-invasive method and applied easily to determine the cardiac functions and its abnormalities in cattle. This chapter deals with the anatomy and position of the heart in the thoracic cavity in cattle. Describes the indications, precautions and requirements for ECG in cattle. Positioning of the animals and methods to be followed for ECG examination. Guidelines to be followed to read the ECG graph, normal waves and values of ECG and interpretation of ECG in different diseases conditions. ECG is an aid in the diagnosis of diseases like atrial enlargement, ventricular hypertrophy, anemia, mineral and electrolyte imbalance, myocardial infarction and classification of cardiac arrhythmias.