The mechanisms of disorders in cell functions induced by 1,4-naphthoquinone amide derivatives are not clarified yet. The article is dedicated to the study of features of these substances influence on loach Misgurnus fossilis L. embryos pro/antioxidant homeostasis during early embryogenesis. The aim of this work was to study the effect of 2-chloro-3-hydroxy-1,4-naphthoquinone, 2-chloro-3-(3-oxo-3-(piperidine-1-yl)propylamine)-1,4-naphthoquinone (FO-1), 2-chloro-3-(3-(morpholine-4-yl)-3-oxopropylamine)-1,4-naphthoquinone (FO-2 at concentrations of 10-3, 10-5, 10-7 M on the content of TBA-reactive substances (a byproduct of lipid peroxidation) and the activities of superoxide dismutase and catalase in loach embryos. It was established that 1,4-naphthoquinone amide derivatives and 2-chloro-3-hydroxy-1,4-naphthoquinone decreased the content of lipid peroxidation products in embryo cells in a dose-dependent manner. The investigated compounds cause an increase in superoxide dismutase and catalase activities compared with the control value. The results of the two-factor ANOVA test indicate that 2-chloro-3-hydroxy-1,4-naphthoquinone and 1,4-naphthoquinone amide derivatives (FO-1, FO-2) have predominant influence on the TBA-reactive substances content and superoxide dismutase activity. However, the time of loach embryos development has a more pronounced effect on catalase activity than the studied 1,4-naphthoquinone derivatives.

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У статті наведено результати досліджень ультраструктури зародків в’юна Мisgurnus fossilis L. на стадіях першого та десятого поділів бластомерів у середовищі інкубації з 2-хлоро-3-гідрокси-1,4-нафтохінона та амідними похідними (ФО-1 і ФО-2) у концентраціях 10-5 М та 10-7 М. Дія досліджуваних сполук у концентрації 10-5 М призводить до значних ультраструктурних змін клітинних органел, гіпертрофії гранулярного й агранулярного ендоплазматичного ретикулуму, дезорганізації мітохондрій, підвищення кількості лізосом.

Influence of sodium hypochlorite (SH), histamine and their simultaneous action on structural features of respiratory part of rats lungs on 1 st, 7 th, 14 th day of the experiment and after the rehabilitation period (21st day) was investigated. Histamine has been shown to lead to a dose-dependent decrease in the lumen of the alveolus, increase the thickness of interalveolar walls, bronchial spasm, damage to mitochondria, endoplasmic reticulum, increase in the number of peroxisomes and lysosomes. Giving rats SH at a concentration of 20 mg/l causes dystrophic changes in the lung cells, increase of the connective tissue, reducing the area of the cross-section of the alveolus throughout the duration of this compound, whereas SH at a concentration of 5 mg/l leads to less severe pathological changes. At the simultaneous SH and histamine into the body there is a hydropic dystrophy due to damage to the structures of the endoplasmic reticulum and mitochondria, spasmation of bronchioles, increased surfactant production, and the increased of connective tissue. These changes are less pronounced in the simultaneous action of SH at a concentration of 5 mg/l and histamine in a dose of 8 μg/kg.