Platelets play an important part in the progression and pathological angiogenesis of brain glioma because of the different granules content and release of microvesicles that are the source of numerous mediators and bioactive substances, which probably provides a "strategy" for the tumour survival. The objective of study was exploring the effect of platelet-released secretion products of patients with brain glioma on the experimental model of tumour growth in vitro. For this purpose, the cells of glioma C6 were cultured for 72 hours under the addition of modified media containing platelet-released secretion products or conditioned media of peripheral blood cells of patients with glioma as well as persons of the comparison group without rough somatic pathology. In control glioma C6 cultures in standard conditions cell clusters were formed by the type of "spheroids", from which radial cell migration occurred, a tense cellular or reticular growth zone was formed, and tumour cells preserved their ability to mitotic division. Under the influence of platelet-released secretion products of patients with glioma, differently directed effects on cell mitotic activity and the number of cell clusters in glioma C6 cultures were detected depending on the degree of tumour malignancy: stimulating effect under the influence of platelet factors of patients with high-malignancy glioma (G4) and inhibitory effect – due to the influence of platelet factors of patients with differentiated glioma (G2). In contrast to the thrombocyte-released factors, the conditioned media of a common pool of peripheral blood cells of patients with G4 glioma suppressed the mitotic activity of tumour cells and did not affect the number of cell clusters. No changes in glioma C6 cultures were revealed after the influence of platelet-released secretion products of persons of the comparison group. The obtained data confirm the important role of platelets in the pathogenesis of brain glioma, pointing to the fundamental difference in the spectrum of biologically active molecules that are released by platelets of patients depending on the degree of tumour malignancy and are able to regulate the cell cycle and proliferative activity of the glioma tumour cells, which may have application as a diagnostic marker as well as predictive marker of response to antitumour therapy.

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Common immtmoregulatory carbohydrate receptors on the membranes of neurons and sysngeneic peripheral lymphocytes of mice are detected by using lectins. Brain neuron membranes possess no receptors characteristic of immature lymphocytes. The common immunoregulatory receptors on brain neurons and mononuclear cells of peripheral immune organs are shown to represent one of the mechanisms of integration of the nervous and immune systems. Key Words: carbohydrate lectin receptors; neurons; lymphocytes; immunoregulationAccording to current views, many immunoregulatory effects: proliferative, suppressor, and contrasuppresser, are mediated through the binding, expression, or hindrance of certain carbohydrate determinants of lymphokines and membrane receptors of immunocompetent cells by sialic acids [3][4][5]8]. Several types of carbohydrates, namely D-galactose, D-mannose, N-acetylglucosamine, N-acetylgalactosamine, a-fucose, and neuraminic acid, are known to be typically involved in various regulatory reactions between cells [3]. It is known, in particular, that the proliferative response of lymphocytes is primarily induced by mannose-specific receptors, starting from the production of IL-1 by macrophages whose activity is determined by the expression of D-mannose [9], with the subsequent production of IL-2, another mannose-specific glycoprotein, by T lymphocytes [11], and including the production and secretion of IgM, where D-mannose is a constituent of the carbohydrate core of the Accumulated experimental data imply the commonness of immunoregulatory receptors on cell membranes in the nervous and immune systems. The identification of common immunoregulatory receptors on neurons and peripheral blood mononuclear cells may provide a basis for deliberate modification of their structure for the creation of new preparations and methods of diagnosing and treating various CNS disorders. In this connection, the aim of the present investigation was a parallel study of the expression of lectin receptor on neurons and syngeneic lymphocytes in experimental mice in order to clarify one of the mechanisms of integration of the nervous and immune systems. MATERIALS AND METHODSBrains of C57B1/6 mice were aseptically removed into Eagle medium, dissected with a needle, and left at room temperature during 1 hour. Then the

1035tumor and native cells and between the number of type I and II epithelial cells in affected and unaffected tissue, These findings point at homeostatic stability of epithelial-mesenchymal structures in breast carcinoma, prompting the search for new approaches to its regulation.The content of the D-mannose-specific LCL-receptor that binds D-mannose-containing lymphokines (interleukin-1 and interleukin-2) is proportional to the degree of glioma anaplasia. This may reflect the mechanism whereby brain glioma utilizes lymphokines to proliferate and to escape immunologic surveillance.