The main aim of this research was to establish the nucleotide sequence of the highly variable region of the D loop of the mitochondrial DNA of some Georgian domestic animal species (cattle, goat, sheep) as well as their phylogenetic position among the worldwide set of domestic animals. In this study, a total of 5 haplogroups (T – 5; T3 – 7; T1 – 1; T2 – 2; T5 – 2) in 17 Georgian Mountain cattle (GMC), 4 haplogroups (A – 15; A2a1 – 3; A1a – 1; A6 – 3) in 22 Georgian goats and 3 haplogroups (A – 10; B – 16; C -15) in 41 Georgian sheeps (15 Imeretian and 26 Tushetian) were detected. This study represents the first attempt of Genetic study of native Georgian livestocks. The GMC, Georgian (Megrelian) goat, Georgian (Imeretian and Tushetian) sheep mitogenomes were grouped phylogenetically in the haplogroups indicating the closeness to the Near Eastern animals.

Grapevine is the one of the most important fruit species in the world. Comparative genome sequencing of grape cultivars is very important for the interpretation of the grape genome and understanding its evolution. The genomes of four Georgian grape cultivars-Chkhaveri, Saperavi, Meskhetian green, and Rkatsiteli, belonging to different haplogroups, were resequenced. The shotgun genomic libraries of grape cultivars were sequenced on an Illumina HiSeq. Pinot Noir nuclear, mitochondrial, and chloroplast DNA were used as reference. Mitochondrial DNA of Chkhaveri closely matches that of the reference Pinot noir mitochondrial DNA, with the exception of 16 SNPs found in the Chkhaveri mitochondrial DNA. The number of SNPs in mitochondrial DNA from Saperavi, Meskhetian green, and Rkatsiteli was 764, 702, and 822, respectively. Nuclear DNA differs from the reference by 1,800,675 nt in Chkhaveri, 1,063,063 nt in Meskhetian green, 2,174,995 in Saperavi, and 5,011,513 in Rkatsiteli. Unlike mtDNA Pinot noir, chromosomal DNA is closer to the Meskhetian green than to other cultivars. Substantial differences in the number of SNPs in mitochondrial and nuclear DNA of Chkhaveri and Pinot noir cultivars are explained by backcrossing or introgression of their wild predecessors before or during the process of domestication. Annotation of chromosomal DNA of Georgian grape cultivars by MEGANTE, a web-based annotation system, shows 66,745 predicted genes (Chkhaveri-17,409; Saperavi-17,021; Meskhetian green-18,355; and Rkatsiteli-13,960). Among them, 106 predicted genes and 43 pseudogenes of terpene synthase genes were found in chromosomes 12, 18 random (18R), and 19. Four novel TPS genes not present in reference Pinot noir DNA were detected. Two of them-germacrene A synthase (Chromosome 18R) and (-) germacrene D synthase (Chromosome 19) can be identified as putatively full-length proteins. This work performs the first attempt of the comparative whole genome analysis of different haplogroups of Vitis vinifera cultivars. Based on complete nuclear and mitochondrial DNA sequence analysis, hypothetical phylogeny scheme of formation of grape cultivars is presented.

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