. The 4% extract generated optimum number of leaves and 1% extract was the best concentration to produce highest length of stem powder and extract effectively improved shoot . But, the extract showed faster effect on C.growth than the powder. The phytochemical screening showed positive result for the steroid. This result supported and shed light on how the Z.C. nutans. This study site large scale field application.vitro; phytochemicals; shoot propagation; Zea mays stem.