Ethanol production from corn grain is taken a point of convergence as an alternative fuel development recently. Herein, we studied the production of ethanol from Zea maize L with designing the production plant and optimization process, respectively. As an additional source, the maize grains are processed to a fine flour and then exposed to liquefaction, where the temperature was maintained at 85 ° C for 4 hours. Furthermore, it was exposed to saccharification for 4 hours at 50°C with subsequent refining. The ethanol yield acquired from the process was observed to be 10.6, with the purity of 95%. The distinctive ethanol properties are likewise evaluated with the specific gravity, relative thickness and the refractive index were 0.79, 0.85, and 1.4, respectively.