The paper shows the results of biochemical analysis of the protein status of Kemerovo pigs. The research was carried out at OOO Chistogorskiy in Kuzbass. The blood samples from pigs were taken by means of the standard methods and analyzed in the laboratory of veterinary genetics and biochemistry of Novosibirsk State Agrarian University. The researchers used standard tools for biochemical tests on the biochemical semi-automatic analyzer Photometer 5010 V5+. The concentration of some protein metabolic parameters was characterized by a standard distribution: total protein (p-value=0.85), albumin (p-value=0.06), and globulin (p-value=0.65). The average values of total protein and albumins varied in standard intervals, except for the level of globulins, which was significantly lower than the reference values set for pigs. It was seen as one that had the greatest individual fluctuations (44%). The urea concentration was slightly lower than the physiological standard and was characterized by a rather high level of individual variability (50%). The researchers found that uric acid concentration was 3.2 times lower than the standard one in the investigated pigs. The authors observed the interbred differences in the content of total protein and albumin comparing the Kemerovo, Chistogorsk and early maturing pig breeds (CM-1). The correlation analysis has shown that there is a high positive correlation between total protein and globulins (r=0.88). The average positive relation between urea and uric acid was (r=0.58). The obtained data on protein metabolism is one of the basic characteristics of the Kemerovo pigs interior.

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In the article, the authors conducted a comprehensive study of the interior, including biochemical, physiological, physicochemical, cytogenetic, and other indicators in sheep of the Romanov breed of Western Siberia. For the study, blood serum samples were taken from 10 rams-producers and 30 lambs at four months. Conventional methods took blood samples. Analyses were performed in the biochemistry laboratory of Novosibirsk State Agrarian University using the technique of competitive enzyme immunoassay with the reagent kit “SteroidIFAtestosterone” for the quantitative determination of testosterone concentration in blood serum. In the sheep breeding area, the authors constantly monitored the content of heavy metals in water, soil, feed, organs, and tissues. The concentration of chemical elements did not exceed the permissible exposure limit. Enzyme-linked immunosorbent assay was performed on a Thermo Scientific Multiskan FC analyzer. The authors performed statistical processing of experimental data using standard methods of descriptive statistics, the statistical programming language, or the RStudio data analysis environment (using the functions DescrStats, Summary, Sd, Read. Table, Write. Table, Aes). The authors established the influence of the age of ontogenesis on the testosterone content in Romanov rams. Presented figure with two variation curves with high transgression. The average testosterone level was 1.8 times higher in lambs (0.774 mmol/l) than in lambs. Testosterone concentration was characterized by high phenotypic variability. The authors have identified reference intervals for testosterone in blood serum depending on environmental conditions in sheep of the Romanov breed of Western Siberia.

There is practically no statistical analysis of the influence of the degree of inbreeding on the reproductive qualities of laboratory mini-pigs in the literature. The present work is aimed at determining the impact of the inbreeding factor on the development of such signs of reproduction as live weight at birth, multiple births and the safety of piglets during the suckling period. Laboratory mini-pigs bred at the Institute of Cytology, and Genetics SB RAS (Novosibirsk, Russia) were selected as the research object. The following signs were directly evaluated: the mass of an individual at birth (significant fertility); intra-nest minimum and maximum by live weight at birth; the intra-nest standard deviation of live weight at birth; the number of live piglets in the nest at birth (multiple births); safety from birth to 30 days; safety from the 6th to the 30th day of life. The results showed that the relationship between Shapiro and the inbreeding coefficient by Wright did not significantly affect the studied traits. The use of a linear regression model in the analysis indicates the existence of an inverse dependence of the intra-nest standard deviation of the live weight of a newborn piglet on the Wright inbreeding coefficient. An assumption was made explaining the almost complete absence of the influence of the inbreeding coefficient on the development of the average values of reproductive traits. This was because of the breeding system, in which close inbreeding was used with “looping” on the best animals. The primary vector of selection is aimed at the viability and development of a harmonious exterior of animals. Thus, systematic closely related crosses with the primary vector contributed to the purification of the herd from harmful recessive mutations, which are considered the leading cause of the negative consequences of inbreeding