Soybean demand from year to year is increasing in line with an increase in population and increasing people’s understanding of nutritious food, while soybean production is still very low. Therefore, various efforts continue to be made to increase soybean production, for example by selecting high-yielding varieties and improving soil fertility. This research was conducted with the aim of increasing soybean crop production by selecting appropriate varieties and improving soil fertility by application mycorrhizal biofertilizers. This research was conducted in the form of experiments in the district of Takalar, South Sulawesi, which took place from April to July 2019. The experiment was designed with the Split Plot Design. As the main plot is the treatment of varieties consisting of 3 types, namely: Anjasmoro varieties, Argomulyo varieties and Gema. As a subplot is the provision of mycorrhizae consisting of two levels, namely without the application of mycorrhizae and mycorrhizal applications. From these two factors, 6 treatment combinations were obtained and were repeated three times, so that 18 experimental units were obtained. The results showed that there was an effect of varieties treatment and mycorrhizal application on plant growth and soybean crop production. The use of Gema varieties and their interactions with the application of mycorrhizae produce the highest soybean plants. The parameters of production components and soybean production indicate that mycorrhizal application produces the highest number of productive branches, while the use of Anjasmoro and Argomulyo varieties produces the highest number of pods, pod weight and dry seed production per ha.