Many beneficial medicinal products are used to treat various serious diseases and disorders like diabetes, cancer and cardiovascular diseases without side effects. Hence our study focused to investigate the phytochemical analysis, quantification of bioactive compounds (alkaloid, phenols, flavonoids), in vitro free radical scavenging activity, anti-inflammatory activity (Membrane stabilization assay), in vitro anti-diabetic activity by enzyme inhibition activity, glucose uptake assay in isolated rat hemi-diaphragm and in yeast cells and also record GC-MS profile of Erythrina variegata L. bark (methanolic extract) which has boundless medicinal properties. The results of this study showed the evidence that the extracts when tested for their phytochemicals and free radical scavenging activity were found to have considerable antioxidant potential. This plant also exhibit better in vitro enzyme inhibitory activity, inhibition of non-enzymatic glycosylation of hemoglobin, in vitro anti-diabetic activity proved by glucose uptake assays and anti-inflammatory activity. GC-MS analysis of methanolic extract of Erythrina variegata L. bark revealed the presence of 58 compounds which are responsible for pharmacological activities. The results of this study indicate that the methanolic extract of Erythrina variegata L. bark has significant pharmacological properties.

GC-MS is one of the best techniques to identify the constituents of volatile matter, long chain, branched chain hydrocarbons, alcohols acids, esters etc. The freshwater mussels Parreysia corrugata was analyzed using Gas Chromatography–Mass Spectrometry, while the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. Gas chromatography mass spectrometry (GC-MS) analysis revealed the presence of 26 compounds. The compounds were identified by comparing their retention time and peak area with that of literature and by interpretation of mass spectra. The first compound identified with less retention time (30.236 min) was 2,6-Difluorobenzoic acid, tridec-2-ynyl ester, whereas gamma.-Tocopherol was the last compound which took longest retention time (29.84min) to identify. Many of them are used in industry for various applications like flavor, antioxidant, anti-inflammatory, antimicrobial, pesticide and cancer preventive. Keywords: Freshwater mussels, Parreysia corrugata, GC-MS, Bioactive components