Background and Aim: Natural resistance-associated macrophage protein 1 encoding gene (Nramp1) plays a role in immune response and disease resistance. This study aimed to investigate the polymorphisms of Nramp1 intron 6 concerning Salmonella shedding and hematological traits in pigs. Materials and Methods: A total of 40 commercial pigs (three-way Large White x Landrace x Duroc cross) were genotyped using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and analyze the relationship between the polymorphisms of the Nramp1 gene and Salmonella fecal shedding and hematological parameters. Results: Nramp1 was shown to be polymorphic in these pigs. The Nramp1 gene has two alleles (A and B) and two genotypes (AB and BB). The BB genotype had a higher frequency than the AB genotype. A significant relationship between the BB genotype and the number of Salmonella in feces compared to the AB genotype (p < 0.05) on 7 days post-inoculation (DPI) was revealed in the association analysis. The single-nucleotide polymorphism at intron 6 in the Nramp1 gene was linked to white and red blood cells 2 and 7 DPI (p < 0.05). Conclusion: The Nramp1 gene was suggested by these findings to be potentially used as a molecular marker for the genetic selection of disease susceptibility in pig breeding.