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To achieve the desired mechanical and biological properties, the weight percentage of each component in bioactive glass/chitosan/zeolite bio-composite fabricated via the liquid phase method was optimized. In the first step: the weight percentage of zeolite was kept constant and the amounts of the other two components were modified considering the mechanical properties. In the second step, to optimize the weight percentage of the zeolite, the best sample of the first step was re-fabricated with different zeolite percentages (5 to 20 wt.%). All the bio-composites were identified by FT-IR, XRD, FE-SEM, and EDS, then their mechanical properties were measured. To determine the biological activity, samples were immersed in SBF for 1, 3, and 7 days and the hydroxyapatite growth rate was measured by XRD. Results specified that the sample with 65 wt.% bioactive glass/20 wt.% chitosan/15 wt.% zeolite had the best mechanical properties and had excellent hydroxyapatite growth in three days.