Abstract. The purpose of this research was to determine the kinetic parameters for flooded soil recovery via soil containing Arbuscular Mycorrhizal fungi (AMF). The general procedures of this experiment started by preparation of simulated flooded soil (FS) and soil containing AMF (SA). Mixed soil was prepared by mixing FS and SA with ratio 1:1. Onion plant was chosen as a host plant and planted in the mixed soil for 14 days. The plantation was conducted in ambient temperature. The nutrients (nitrogen, phosphorus and potassium) concentrations in the soil were tested using HACH Spectrophotomer. The Michaelis-Menten equation was used to study the nutrients recovery in soil. The Lineweaver-Bulk plot was used to solve the Michaelis-Menten equation. From the experiment conducted, the maximum nutrient uptake (Vmax) and bonding affinity (Km) obtained for nitrogen (N) were 6.28mg/l.d and 82.17 mg/l, for phosphorus (P) were 9.80 mg/l.d and 60.96 mg/l.d and for potassium (K) were 0.07mg/l.d and 4.55mg/l. By comparing the result with other researcher, it showed that the Vmax and Km of nitrogen (N) and phosphorus (P) obtained were higher than other research. This was because the onion required a high level of N and P in the soil compared to other host plant.
Abstract. This paper presents the factorial analysis on flooded soil (FS) recovery using soil containing Arbuscular Mycorrhizal fungi (SA) with the help of a selected host plant, Allium cepa L. (onion plant). In order to study the most contributing factor for the soil recovery, five factors were selected namely; pH of soil, water content, SA/FS ratio, light supply and depth of soil. This was to study the ability of SA to recover the nutrients (nitrogen, phosphorus and potassium) in the FS. Experimental design table was constructed using the concept of two levels factorial by employing DesignExpert® software (Ver 7.1.6). Soil sample was collected after 12 days and analyzed for nitrogen, phosphorus and potassium concentrations using HACH Spectrophotometer. Later, the data was analyzed in the named software to determine the main effect of respective factors and interaction between factors. The results showed that water content, light supply and depth of soil were the important factors for nutrients recovery. The best condition for FS recovery using SA was at: pH of the soil (4), water content (28 ml), SA/FS ratio (1:5), depth of soil (5 cm) and with light supply.
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