The results of the study of lymphoid organs and sera of wild boars for the presence of DNA of African swine fever (ASF) virus and RNA of classical swine fever (CSF) virus are presented, as well as the results of a serological examination for the presence of ASF and CSF virus antibodies. The study was conducted in Ukraine between 2008 and 2013. Biological samples were obtained from wild boars shot during the hunting season, and were examined by real-time PCR and ELISA. In total, 5759 sera were tested for CSF virus antibodies and 4856 for ASF virus antibodies by ELISA. Samples of lymphoid organs totalling 1129 were examined by PCR for the detection of CSF virus RNA and 8102 such samples were examined for the detection of ASF virus DNA. CSF virus antibodies were detected in 6.56% of wild boar sera. RNA of CSF virus was also identified in 1 out of 1129 samples tested. ASF virus antibodies or DNA in lymphoid organ samples were not detected.

У статті наведені результати досліджень щодо виділення 10 ізолятів Streptococcus suis та їх ідентифікації за бактеріоскопічними, культуральними, біохімічними та біологічними властивостями. Встановлено, що серед ізолятів Streptococcus suis найвищий рівень вірулентності (LD50) до білих мишей був притаманний штамам «3/2» (187±28 КУО/см 3 ), «21» (225±40 КУО/см 3 ), «10» (380±48 КУО/см 3 ) та «19» (891±63 КУО/см 3 ) за середнього розрахунку на 1 дослідну тварину. Аналіз результатів досліджень з визначення локалізації збудника Streptococcus suis в тканинах організму білих мишей після їх зараження показав, що найчастіше збудник локалізувався у печінці (80%), селезінці і головному мозку (60%). Із крові збудник виділено у 82,5% випадків від усіх досліджених зразків. Найменша частота виділення Streptococcus suis спостерігалась за дослідження зразків легеньдо 17,5 % від усіх дослідних зразків.

No abstract

This study presents novel predictive equations for von Mises stress values of bones in the frontal and lateral regions of the skull. The equations were developed based on results of a finite element model developed during this research. The model was validated for frontal and lateral loading conditions with input values mimetic to fall scenarios. Using neural network processing of the information derived from the model achieved R 2 values of 0.9990 for both the stress and deflection. Based on the outcome of the fall victims, a threshold von Mises stress of 40.9 to 46.6 MPa was found to indicate skull fracture given a maximum input force of 26 kN and a load rate of 40 kN/ms.

The bacteriological study of sausages was carried out under conditions of agro-food markets and supermarkets in Odessa. It was established that the bacteriological contamination of sausages that did not meet the veterinary and sanitary requirements by the standard plate count in supermarkets ranged from 2.5×10 3 to 1.0×10 4 CFU per 1 g, which was 18.0% of 44 samples, and in the markets from 2.7×10 3 to 1.5×10 4 , which was 29.4% from 34 samples. The most contaminated with the coliform bacteria were cooked sausages of all grades -41% of the samples of all kinds sausages.