The aim: To determine the degree of severity of the inflammatory process in periodontal tissues of children living in different ecological conditions. Materials and methods: With the purpose of estimation of the condition of periodontal tissues the epidemiology examination of 642 children that live on territory with the high level of pollution and natural deficit of iodine and fluorine was conducted. Results: The study found that the increase of age of examined children causes the decrease of percentage of easy degree of severity of chronic catarrhal gingivitis, giving way to the advanced forms of the disease, and this process was more pronounced in children living in ecologically polluted region. In children-inhabitants of ecologically clean region easy degree of severity of chronic catarrhal gingivitis met more often than their peers living in environmentally polluted region. At the same time, average and heavy degree of severity of chronic catarrhal gingivitis in children living in ecologically contaminated region met more often than their peers, inhabitants of ecologically clean region. Conclusions. It was found that with increasing age of the examined children decreases the percentage of chronic catarrhal gingivitis of moderate severity.

149синтазной системы и развитие воспалительных процессов у крыс разного возраста в динамике отравления табачным дымом. Исследования проведены на белых беспородных крысах-самцах разного возраста, которые ежедневно в течение 45 дней подвергались воздействию табачного дыма в герметичной камере (время экспозиции 6 мин). Через 15, 30 и 45 суток от начала поражения животных табачным дымом, их выводили из эксперимента путем эвтаназии под тиопенталовым наркозом с соблюдением всех правил по работе с позвоночными животными. Установлено, что поражение крыс в течение 45 дней табачным дымом привело к увеличению в сыворотке крови содержания провоспалительного цитокина IL-6. Наиболее чувствительными к данному токсиканту оказались неполовозрелые крысы, в которых содержание IL-6 увеличилось на 50%, 164% и 237% соответственно через 15, 30 и 45 дней после поражения. Отравление крыс табачным дымом привело к повышению активности индуцибельной NO-синтазы в сыворотке крови и печени в течение всего срока исследования. Отмечено, что организм неполовозрелых крыс наиболее быстро и наиболее выражено реагирует на табачный дым. Активность іNO-синтазы наибольших значений достигла как в сыворотке крови, так и в печени неполовозрелых животных. После поражения крыс табачным дымом в наших экспериментах отмечалось снижение активности эндотелиальной NO-синтазы во всех возрастных группах крыс. У неполовозрелых крыс активность еNOS в сыворотке крови снизилась в 1,4 раза, 1,8 и 2 раза через 15, 30 и 45 дней соответственно по сравнению с нормой. В печени крыс всех возрастных групп в течение эксперимента наблюдалось аналогичное снижение активности еNOS и наименьшего значения она достигла в последний срок исследования (45 день поражения табачным дымом).Ключевые слова: табачный дым, белые крысы, нитроокидативний стресс, NO-синтаза, интерлейкины.the NO-synthase system and the development of inflammatory processes in rats of different ages in the dynamics of tobacco smoke poisoning. Studies were carried out on white, non-native male rats of different ages, which were exposed to tobacco smoke daily for 45 days in a sealed chamber (exposure time 6 min). After 15, 30 and 45 days from the onset of the animals' defeat with tobacco smoke, they were taken out of the experiment by euthanizing them under thiopental anesthesia, observing all the rules for working with vertebrates. It was found that the damage to the rats for 45 days with tobacco smoke led to an increase in the serum content of the proinflammatory cytokine IL-6. The most sensitive to this toxicant were immature rats, in which the content of IL-6 increased by 50%, 164% and 237%, respectively, 15, 30 and 45 days after injury. Poisoning of rats with tobacco smoke led to an increase in the activity of inducible NO-synthase in serum and liver throughout the study period. It is noted that the body of immature rats reacts most quickly and most strongly to tobacco smoke. The activity of iNO synthase reached the highest values both in the blood serum and in the liver of immature animals. After the defeat of ra...

No abstract

No abstract

The aim of the study was to assess the state of oral liquid (OL) immunity in children with chronic catarrhal gingivitis (CCG) living in adverse environmental conditions. 120 children aged 7-15 residing in ecologically unfavorable areas of Lviv region were examined, while 75 children living in 'relatively clean' region were involved in the control group. Chronic catarrhal gingivitis was diagnosed according to Danilevskiy classification (1994). The level of cytokines in oral liquid of 7-years-old children living in ecologically polluted region (EPR), was (198.19±4.11)·106/l, which was 1.4 times more than in the conditionally clean region (CCR): (141.09±4.10)·106/l (p<0.01). Analysis of cytokine profile in 7-years-old from EPR showed increased levels of IL-6 proinflammatory cytokines by 11.22% (13.78±0.38 pg/ml vs 12.39±0.50 pg/ml in controls, p<0.05) and the decrease of IL-4 anti-inflammatory cytokine by 26.9% (7.12±0.62 pg/ml vs 9.74±0.58 pg/ml, p<0.01). In 12 years-old from EPR quantity of leukocytes in OL was 1.3 times higher than in controls ((246.81±4.16)·106/l vs (190.02±4.11)·106/l, p<0.01), the increase of the IL-6 content of 27.1% (p<0.01) and reduce of the IL-4 of 21.5% (p<0.05) compared to controls was also seen. At the age of 15 further increase of leucocytes in children from EPR was revealed: (297.53±4.15)·106/l, which was 1.2 times higher than in controls (p<0.01). Changes of cytokine profile in this age group were characterized by increased content of IL-6 of 26.41% (p<0.05) and IL-4 drop of 28.53% (p>0.05). Thus the age-dependent trend for the increase of leukocytes count in OL and pro-inflammatory cytokine IL-6 with the decrease of anti-inflammatory cytokine IL-4 is noted in children with CCG living in EPR.