line) • Біологічні Студії / Studia Biologica • 2018 • Том 12/№3-4 • С. 3-16 waste dumps. The obtained results allow concluding about the wide distribution of representatives of the Acidi thiobacillus genus in the dumps of the fuel-energy complex, their biological diversity, and a possibility of their use in biotechnologies of metal leaching.

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Today both academic and commercial interests in marine actinobacteria are growing. As they live in a unique environment that promotes the synthesis of new biologically active metabolites. The aim of the work is isolation, primary identification and study of morphological, cultural, physiological and biochemical properties of actinobacteria, separate from mussels (Mytilus galloprovincialis) of the Odessa Bay of the Black Sea. Methods. Samples of mussels collected in the coastal zone of Odessa Bay were used as material for the isolation of actinobacteria. Isolation of actinobacteria and the study of their morphological, cultural, physiological and biochemical properties was carried out by traditional microbiological methods. The fatty acid composition was determined on an Agilent 7890 semi-ionization gas chromatograph (Agilent Technologies, USA) to identify test strains using the Sherlock Microbial Identification System library. Results. From samples of mussels collected in 2020 in the area of the Hydrobiological Station of Odessa National I.I. Mechnikov University were isolated 14 strains of actinobacteria. They were identified by fatty acid analysis to Streptomyces genus. Strains of Streptomyces sp. Myt2, Myt6 and Myt7ch synthesized melanoid pigments. Strains of actinobacteria are well absorbed by most of the studied sources of carbon, except strains of Streptomyces sp. Myt12a, Myt12b. Almost half of the strains have oxidase activity and coagulate milk. Conclusions. As a result of microbiological studies for the first time were characterized morphological, cultural and physiological and biochemical properties and determined taxonomic composition by fatty acid spectra for actinobacteria isolated from the mussels of the Odessa Bay of the Black Sea.

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Evaluation of Lactobacillus plantarum strains perspective for plant protection or fermentation of food of plant origin should include tests on abilities to attach to plant surfaces and to form biofilms. Comparing RAPD-profiles with these properties and also with geographical origin of the strains is necessary to find out the possibility of rapid selection of the strains and tracking their dissemination. Biofilm formation and RAPD-PCR profiles of 30 strains of L. plantarum isolated from grape must and pickles originated from France and Ukraine were compared. All isolates were able to attach to Lepidium sativum seedlings and form biofilms of different types. Amount of attached cells varied from (7.8 ± 0.2) x 104 to (2.1 ± 0.3) x 103 CFU/cm2. The most developed biofilms were formed on shoots, leaves, seed coat shells. On roots this ability was strain dependent: the majority of French isolates (77.0%) formed separated microcolonies without developed matrix layer. Among Ukrainian isolates, 47.1% of the strains showed the same tendency, and in 41.2% of the strains individual attached cells without formation of microcolonies were observed. The level of initial attachment of bacterial cells to roots did not coincide with the level of subsequent formation of biofilms. Gene plnA was detected in 28.1% of isolates. All plnA possessing strains except the one formed microcolonies or biofilms with developed matrix. Lactobacilli could eliminate the biofilms of Agrobacterium tumefaciens pJZ from tomato roots in 75.0 – 100% of tested samples depending on a strain and method of inoculation. No clear association between the geographical origin of the L. plantarum strains and clustering by RAPD-PCR analysis was found. Two strains with high level of biofilm formation were grouped in one subcluster.