Herein, the use of red blood cells (RBCs) as carriers of cytoplasmically interned phototherapeutic agents is described. Photolysis promotes drug release from the RBC carrier thereby providing the means to target specific diseased sites. This strategy is realized with a vitamin B12‐taxane conjugate (B12‐TAX), in which the drug is linked to the vitamin via a photolabile CoC bond. The conjugate is introduced into mouse RBCs (mRBCs) via a pore‐forming/pore‐resealing procedure and is cytoplasmically retained due to the membrane impermeability of B12. Photolysis separates the taxane from the B12 cytoplasmic anchor, enabling the drug to exit the RBC carrier. A covalently appended Cy5 antenna sensitizes the conjugate (Cy5‐B12‐TAX) to far red light, thereby circumventing the intense light absorbing properties of hemoglobin (350–600 nm). Microscopy and imaging flow cytometry reveal that Cy5‐B12‐TAX‐loaded mRBCs act as drug carriers. Furthermore, intravital imaging of mice furnish a real time assessment of circulating phototherapeutic‐loaded mRBCs as well as evidence of the targeted photorelease of the taxane upon photolysis. Histopathology confirms that drug release occurs in a well resolved spatiotemporal fashion. Finally, acoustic angiography is employed to assess the consequences of taxane release at the tumor site in Nu/Nu‐tumor‐bearing mice.
Arthritis is a leading cause of disability in adults, which can be intensely incapacitating. The location and intensity of the pain is both subjective and challenging to manage. Consequently, patient-directed delivery of anti-inflammatories is an essential component of future therapeutic strategies for the management of this disorder. The design and application of a light-responsive red blood cell (RBC)-conveyed dexamethasone (Dex) construct that enables targeted drug delivery upon illumination of the inflamed site is described. The red wavelength (650 nm) responsive nature of the phototherapeutic is validated using tissue phantoms mimicking the light absorbing properties of various skin types. Furthermore, photoreleased Dex has the same impact on cellular responses as conventional Dex. Murine RBCs containing the photoactivatable therapeutic display comparable circulation properties as fluorescently labeled RBCs. In addition, a single dose of light-targeted Dex delivery is fivefold more effective in suppressing inflammation than the parent drug, delivered serially over multiple days. These results are consistent with the notion that the circulatory system be used as an on-command drug depot, providing the means to therapeutically target diseased sites both efficiently and effectively.
Annotation. Ischemic heart disease (IHD) is one of the main causes of high mortality, disability and a decrease in the quality of life of the working-age population (WAP) in Ukraine. Aim of the work: to determine the modern features of mortality, disability, prevalence and incidence of ischemic heart disease in the working-age population of the Kyiv region. The analysis of the epidemiology of IHD in the Kyiv region was carried out in dynamics for 2010–2019 and compared to Ukraine. The statistical data of the State Statistics Committee of Ukraine, the Center for Medical Statistics of the Ministry of Health of Ukraine, and the Kyiv Regional Center for Medical Statistics on mortality, disability, prevalence and incidence of IHD in the working-age population of the Kyiv region were analyzed with the medical-statistical method. It has been established that IHD is a significant cause of the loss of labor potential in the Kyiv region. IHD accounts for every fourth (25.0%) death in the working age, and the regional level of working-age population mortality from IHD is the highest in Ukraine. In the Kyiv region working-age population dies from ischemic heart disease 1.5–1.7 times, and from myocardial infarction – 1.7–1.9 times more often than the average in Ukraine. Regional levels of incidence IHD among working-age population exceed similar indicators in Ukraine by 1.2 times, the incidence of myocardial infarction – 1.5 times, the prevalence of ischemic heart disease – 1.7 times. Identified unfavorable dynamic trends in mortality and prevalence of IHD among the working-age population of the Kyiv region. The mortality rate from IHD among working-age population increased in the Kyiv region in 2019 compared to 2010, by 7.9%. The prevalence of IHD increased during 2010–2018 by 14.4%, and the primary incidence of myocardial infarction – by 14.9%. The unfavorable epidemiological situation with IHD among working-age population in the Kyiv region necessitates improving the system for the prevention and the organization of medical care for patients with IHD in this region.
ГБОУ ВПО Красноярский государственный медицинский университет имени проф. В. Ф. Войно-Ясенецкого Министерства здравоохранения РФ, ректор-д. м. н., проф. И. П. Артюхов; кафедра внутренних болезней № 1, зав.-д. м. н., проф. С. Ю. Никулина. p%'>,%. В обзоре представлены литературные данные, свидетельствующие о генетической детерминированности ревматоидного артрита. Дано определение указанной патологии, описаны основные признаки заболевания, рассмотрены гены, влияющие на развитие ревматоидного артрита и их полиморфизмы. j+>7%";% 1+." : ревматоидный артрит, гены ревматоидного артрита.
Мета: оцінити стан здоров'я дорослого населення зони спостереження об'єктів атомної енергетики України на прикладі Запорізької АЕС. Матеріали та методи. Системно оглядовий, бібліографічний, аналітичний, соціологічного опитування та ста тистичний методи. Результати. Встановлено зростання захворюваності дорослого населення м. Нікополь Дніпропетровської об ласті, що входить до зони спостереження Запорізької АЕС, на хвороби органів травлення. Найбільше зростання рівнів захворюваності відзначалося на виразку шлунку та дванадцятипалої кишки, гастрити і дуоденіти, холе цистити, холангіти на 340 %, 305 % та на 83 %, відповідно. Висновки. У зв'язку з проживанням у промислово розвинутому районі і подовження роботи діючих АЕС після закінчення проектного терміну їх експлуатації в Україні, можливим впливом шкідливих чинників на здоров'я на селення зон спостереження, зростанням захворюваності дорослого населення на хвороби органів травлення, стан його здоров'я потребує постійного спостереження та детального вивчення. Ключові слова: атомні електростанції, радіаційний фактор, зона спостереження, громадське здоров'я.
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