Rejasa contains bioactive compounds.Production of bioactive compounds can be done through callus cultures. This study will examine rejasa callus the growth of in various types and concentrations of growth regulators. The independent variables of this study are the types and concentrations of growth regulators (2,4-dichlorophenoxyacetic acid (2,4-D) and Picloram). Dependent variable is growth of callus. Callus growth was determined from the percentage of explants that produced callus, time of callus formation, and callus morphology. The explants used were young petioles grown on Murashige& Skoog solid medium with the addition of 2,4-D and Picloram at various concentrations. The results showed that the lowest percentage of callus formation observed in explants grown on medium with addition of 3.5 ppm 2,4-D (27%). The explants grown on medium with the addition of 3.5 ppm picloram showed the highest callus growth percentage (93%). Explant grown on MS medium supplemented with3.5 picloram showed the best average time of the callus induction, 29.9 days. Callus that is formed mostly brown, and in some treatments produce green callus. Based on the results of this study, the best medium for induction of rejasa callus is MS medium with the addition of 3.5 ppmpicloram.