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An electrochemical sensor for hydrogen peroxide (H 2 O 2) detection was established using 1aminopyrene (AP) as a linker between horseradish peroxidase (HRP) and reduced graphene oxides (rGO) modified glassy carbon electrode. The AP can combine with HRP via a covalent bond and combine with rGO via π-π conjugation. The covalent bond linkages can prevent the enzymes leaching of the enzymes and the π-π conjugate combination can facilitate electrons transfer, which plays synergistic effects to improve the performance of this sensor. The electrochemical activity of the HRP-AP/rGO was described by cyclic voltammetry, alternating current impedance and amperometric techniques. Under the optimal conditions, the biosensor showed a wide linear range from 1.5 μM to 28.5 μM and a low detection limit of 0.5 μM with good stability and high selectivity, confirming that the sensor is well-suited for the detection of H 2 O 2 during biological processes.

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